A modified α-galactosyl ceramide for staining and stimulating natural killer T cells
A modified α-galactosyl ceramide for staining and stimulating natural killer T cells
复制标题
DOI：
10.1016/j.jim.2006.02.009
复制
发表时间：
2006-05-30
影响因子：
2.2
通讯作者：
Savage, Paul B.
中科院分区：
文献类型：
--
作者：
Liu, Yang;Goff, Randal D.;Savage, Paul B.
α -半乳糖神经酰胺在自然杀伤T细胞中的CD1d呈递一直是调节性T细胞研究的焦点。KRN7000是一种α -半乳糖神经酰胺，最初是从海洋海绵糖脂抗肿瘤特性的结构活性研究中产生的，是目前最常用的激动剂配体，用于NKT细胞染色，然而，这种糖脂具有较差的溶解性和可用性。我们开发了一种α -半乳糖神经酰胺，其溶解度优于KRN7000，可有效染色小鼠和人的NKT细胞，并在低浓度下刺激细胞因子释放。(c) 2006 Elsevier B.V.版权所有
CD1d presentation of alpha-galactosyl ceramides to natural killer T cells has been a focal point of the study of regulatory T cells. KRN7000, an alpha-galactosyl ceramide originally generated from structure activity studies of antitumor properties of marine sponge glycolipids, is currently the most commonly used agonist ligand and is used to stain NKT cells, However, this glycolipid suffers from poor solubility and availability. We have developed an alpha-galactosyl ceramide with improved solubility over KRN7000 that effectively stains NKT cells, both mouse and human, and stimulates cytokine release at low concentrations. (c) 2006 Elsevier B.V. All rights reserved.