EFFECTS OF HUMAN RECOMBINANT INTERLEUKINS ON STIMULATION-EVOKED NORADRENALINE OVERFLOW FROM THE RAT PERFUSED SPLEEN
EFFECTS OF HUMAN RECOMBINANT INTERLEUKINS ON STIMULATION-EVOKED NORADRENALINE OVERFLOW FROM THE RAT PERFUSED SPLEEN
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DOI：
10.1007/bf00169139
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发表时间：
1994-05-01
影响因子：
3.6
通讯作者：
FUDER, H
中科院分区：
文献类型：
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作者：
BOGNAR, IT;ALBRECHT, SA;FUDER, H
在离体大鼠脾脏中进行实验，研究淋巴器官中白细胞介素 (IL) 对去甲肾上腺素释放的影响。用 Tyrode 溶液灌注脾脏，通过 HPLC 和电化学检测测定内源性去甲肾上腺素的溢出。血管周围电刺激（4 或 10 Hz，20-28 mA，2 分钟）引起去甲肾上腺素溢出和灌注压力增加，灌注无 Ca2+ 溶液后两者均显着降低，河豚毒素消除，六甲铵不影响，并受α(2)-肾上腺素受体和毒蕈碱受体介导的调节，如萝芙素和醋甲胆碱的作用所示。人重组IL-1β和IL-2以及小鼠重组IL-2 10 ng/ml在暴露时间15分钟后未能影响引起的去甲肾上腺素溢出。相比之下，人重组IL-1β和IL-2 0.1 ng/ml在暴露80分钟后减少了诱发的溢出；尽管进行了清洗，但 30 分钟后抑制作用仍趋于增加。鼠重组 IL-2 1.2 ng/ml 与组织接触 80 分钟后没有引起任何变化，但在冲洗后 30 分钟后出现抑制。人重组IL-6 (0.1 ng/ml) 没有引起显着变化。低浓度IL-1β和IL-2的抑制作用支持免疫系统局部产生的介质可能影响神经元功能的观点。
Experiments were carried out in the isolated spleen of the rat to study in a lymphoid organ the influence of interleukins (ILs) on noradrenaline release. Spleens were perfused with Tyrode's solution and the overflow of endogenous noradrenaline was determined by HPLC with electrochemical detection.Perivascular electrical stimulation (4 or 10 Hz, 20-28 mA, 2 min) caused an increase in noradrenaline overflow and in perfusion pressure, both of which were markedly reduced by perfusion with Ca2+-free solution, abolished by tetrodotoxin, unaffected by hexamethonium, and subject to alpha(2)-adrenoceptor- and muscarinic receptor-mediated modulation as shown by the effects of rauwolscine and methacholine. Human recombinant IL-1 beta and IL-2 and mouse recombinant IL-2 10 ng/ml failed to affect the evoked overflow of noradrenaline after an exposure time of 15 min. In contrast, human recombinant IL-1 beta and IL-2 0.1 ng/ml reduced the evoked overflow after exposure for 80 min; the inhibition tended to increase 30 min later despite washout. Murine recombinant IL-2 1.2 ng/ml caused no change after contact with the tissue for 80 min but there was an inhibition 30 min later after washout. Human recombinant IL-6 (0.1 ng/ml) caused no significant change.The inhibitory effect of low concentrations of IL-1 beta and IL-2 supports the idea that locally produced mediators of the immune system may affect neuronal function.