Invasion of respiratory epithelial cells by Burkholderia (Pseudomonas) cepacia
Invasion of respiratory epithelial cells by Burkholderia (Pseudomonas) cepacia
复制标题
DOI：
10.1128/iai.64.10.4054-4059.1996
复制
发表时间：
1996-10-01
影响因子：
3.1
通讯作者：
Griffith, A
中科院分区：
文献类型：
--
作者：
Burns, JL;Jonas, M;Griffith, A
洋葱伯克霍尔德菌（假单胞菌）引起的肺部感染是囊性纤维化（CF）患者发病率和死亡率的重要原因。提示细胞侵入和B细胞内隔离的几个特征。CF中的头孢菌素是在抗生素治疗面前的持续感染，生物体对抗生素治疗表现出体外敏感性，并倾向于在CF患者中引起菌血症感染。通过改良的庆大霉素保护试验在体外A549细胞中证明了上皮细胞侵袭。侵入的动力学似乎是饱和的。侵入单层的电子显微镜显示胞质内细菌被膜结合的空泡包围。未观察到溶酶体与这些空泡融合。上皮内细胞复制的建议，通过电子显微镜，并证实了定量分析和视觉分析。细胞松弛素D，而不是秋水仙碱，抑制入侵，这表明微丝的作用，但不是微管。B的侵袭表型。洋葱可能是CF感染的重要毒力因子。
Pulmonary infections caused by Burkholderia (Pseudomonas) cepacia are an important cause of morbidity and mortality in cystic fibrosis (CF) patients. Several features suggestive of cellular invasion and intracellular sequestration of B. cepacia in CF are persistence of infection in the face of antibiotic therapy to which the organism demonstrates in vitro susceptibility and a propensity to cause bacteremic infections in patients with CF. Epithelial cell invasion was demonstrated in vitro in A549 cells by a modified gentamicin protection assay. The kinetics of invasion appear to be saturable. Electron microscopy of invaded monolayers showed intracytoplasmic bacteria enclosed by membrane-bound vacuoles. No lysosomal fusion with these vacuoles was observed. Intraepithelial cell replication was suggested by electron microscopy and confirmed by both a quantitative assay and a visual assay. Cytochalasin D, but not colchicine, inhibited invasion, suggesting a role for microfilaments but not microtubules. The invasion phenotype in B. cepacia may be an important virulence factor for CF infections.