Dairyland populations of bur cucumber (Sicyos angulatus) as a possible seed source for riverbank populations along the Abukuma River, Japan
Dairyland populations of bur cucumber (Sicyos angulatus) as a possible seed source for riverbank populations along the Abukuma River, Japan
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DOI：
10.1111/j.1445-6664.2012.00447.x
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发表时间：
2012-12-01
影响因子：
1.4
通讯作者：
Ikeda, Kentaro
中科院分区：
文献类型：
--
作者：
Kobayashi, Hisami;Kurokawa, Shunji;Ikeda, Kentaro
2006年，对刺黄瓜（Sicyos angulatus L.）沿着日本Abukuma河的河岸进行了检查。26个种群被发现在河流上游周围的地区，他们仅限于奶牛场。在对300份材料的叶绿体DNA的16个非编码区的6个多态位点中的4个进行聚合酶链反应-限制性片段长度多态性分析的基础上，鉴定出5种多位点单倍型（AE）。尽管其规模较小，周围地区的人口表现出较高的遗传多样性在单倍型水平比河岸人口。周边地区种群的单倍型组成与上游河岸种群的单倍型组成相似。单倍型D的分布偏向上游，可能是由于周边地区的人口迁移。单倍型E的分布偏向于中下游地区，这与有来源种群而非周边地区种群的存在有关。研究结果还表明，估计的种子流从另一个奶牛场可以运送到中游的种子在一个高的频率，表明奶牛场是主要负责的河岸种群的发展。消灭奶牛场人口应给予优先考虑，以防止未来的迁移沿着河岸和保护现有的濒危湿地物种从入侵S.？有角的。
In 2006, the distribution of bur cucumber (Sicyos angulatus L.) along the river bank of the Abukuma River, Japan, was examined. Twenty-six populations were found in the area surrounding the upper reach of the river and they were limited to dairy land. On the basis of polymerase chain reactionrestriction fragment length polymorphism analysis for four of the six polymorphic loci that were detected in 16 non-coding regions of the chloroplast DNA from 300 samples, five multilocus haplotypes (AE) were identified. Despite its smaller size, the surrounding area's population showed a higher genetic diversity at the haplotype level than did the riverbank population. The haplotype composition of the surrounding area's population was similar to that of the riverbank population in the upper-stream region. The distribution of haplotype D was biased towards the upper reach of the river, possibly because of migration from the surrounding area's population. The distribution of haplotype E was biased towards the middle-to-lower reaches of the river and was associated with the existence of source populations other than the surrounding area's population. The results also showed that the estimated seed flow from another dairy land could transport seeds into the middle reach of the river at a high frequency, suggesting that dairy land is largely responsible for the development of the riverbank populations. The eradication of dairyland populations should be given priority in order to prevent future migration along the river bank and to protect the existing endangered wetland species from invasive S.?angulatus.