Molecular evidence for natural intergeneric hybridization between Liquidambar and Altingia
Molecular evidence for natural intergeneric hybridization between Liquidambar and Altingia
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枫香和Altingia之间自然属间杂交的分子证据
DOI：
10.1007/s10265-009-0275-z
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发表时间：
2010-03-01
影响因子：
2.8
通讯作者：
Shi, Suhua
中科院分区：
文献类型：
--
作者：
Wu, Wei;Zhou, Renchao;Shi, Suhua
自建立以来，基于形态中介和与Altingia和Liquidambar的共面分布，提出了半液丹的杂交起源。然而，这一假说缺乏令人信服的分子证据。为了验证这一假说，本研究测定了位于海南尖峰岭、广东黑石顶和南岭的华夏半潜蝇及其亲本枫香和阿丁香的两个核基因pin2和Cab4和一个叶绿体基因matK的序列。结果表明，台湾乳杆菌与无翅乳杆菌亲缘关系密切，在Pin2和Cab4基因的系统发育树上构成了一个不可分割的分支。系统发育分析显示，华南花的两种序列与其可能的亲本L.formosana-L.聚为一类。Aalycina和A.obovata在尖峰岭的分布，与台湾乳杆菌L.标题黑石顶和南岭的无花果和中华无花果。尖峰岭台湾乳杆菌和倒卵乳杆菌叶绿体matK基因部分序列有4个核苷酸替换，华夏乳杆菌和倒卵乳杆菌两个个体的序列完全一致。在黑石顶和南岭，没有发现包括matK和其他三个叶绿体基因在内的诊断标记来区分台湾乳杆菌和中国乳杆菌。分子生物学数据清楚地表明，华夏血吸虫是台湾血吸虫和台湾血吸虫之间的属间杂种起源。Aalycina和A.obovata或A.chinensis，以及A.obovata在海南尖峰岭的杂交事件中起母本的作用。
Since its establishment, a hybrid origin for Semiliquidambar has been proposed based on morphological intermediacy and sympatric distribution with Altingia and Liquidambar. This hypothesis, however, has lacked convincing molecular evidence. In this study, two nuclear genes, pin2 and cab4, and a chloroplast gene, matK, from Semiliquidambar cathayensis and its putative parental species Liquidambar and Altingia in Jianfengling, Hainan, and Heishiding and Nanling, Guangdong, China, were sequenced to test this hypothesis. Our results showed that L. formosana and L. acalycina were closely related and constituted an inseparable clade in the phylogenetic trees of both pin2 and cab4 genes. Phylogenetic analyses revealed two types of sequences for S. cathayensis, which were clustered with its putative parents, L. formosana-L. acalycina and A. obovata in Jianfengling, and with L. formosana-L. acalycina and A. chinensis in Heishiding and Nanling. The partial chloroplast matK gene sequences showed four nucleotide substitutions between L. formosana and A. obovata in Jianfengling; the sequences of the two individuals of S. cathayensis were identical with those of A. obovata. No diagnostic chloroplast markers including matK and three other chloroplast genes were found to distinguish L. formosana and A. chinensis in Heishiding and Nanling. Molecular data clearly demonstrated that S. cathayensis is of intergeneric hybrid origin between L. formosana-L. acalycina and A. obovata or A. chinensis and that A. obovata functions as the maternal parent in the hybridization event in Jianfengling, Hainan.