Preparation of a molecularly imprinted polymer for the solid-phase extraction of scopolamine with hyoscyamine as a dummy template molecule.
Preparation of a molecularly imprinted polymer for the solid-phase extraction of scopolamine with hyoscyamine as a dummy template molecule.
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DOI：
10.1016/s0021-9673(02)02048-4
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发表时间：
2003-02
期刊：
影响因子：
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通讯作者：
G. Theodoridis;Andreas Kantifes;P. Manesiotis;N. Raikos;H. Tsoukali-Papadopoulou
中科院分区：
文献类型：
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作者：
G. Theodoridis;Andreas Kantifes;P. Manesiotis;N. Raikos;H. Tsoukali-Papadopoulou
以山梨碱(一种紧密的结构类似物)为模板分子，制备了对东莨菪碱具有选择性的分子印迹聚合物(MIP)。生产的聚合物被用作固相萃取的介质，对生物样品中的分析物显示出选择性结合性能。人和小牛的尿液和血清在不同的提取程序下在MIP上处理。在水环境中负载分析物后，通过非选择性疏水相互作用促进在MIP上的保留，可观察到最佳性能。随后使用优化的溶剂系统洗涤分子印迹聚合物，以实现分析物的选择性解吸。其他相关和非相关化合物被访问以评估分子识别性能。生物样品中感兴趣的分析物的回收率高达79%。
Molecularly imprinted polymers (MIPs) selective for scopolamine were produced using hyoscyamine (a close structural analogue) as template molecule. The produced polymers were used as media for solid-phase extraction, exhibiting selective binding properties for the analyte from biological samples. Human and calf urine and serum were processed on the MIP under various extraction protocols. The best performance was observed after loading the analyte in aqueous environment facilitating retention on the MIP by non-selective hydrophobic interactions. The MIPs were subsequently washed using an optimised solvent system to enable selective desorption of the analyte. Other related and non-related compounds were accessed to evaluate molecular recognition properties. Recoveries of up to 79% were achieved for the analyte of interest from biological samples.