Isolation and identification of a novel human parechovirus
Isolation and identification of a novel human parechovirus
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DOI：
10.1099/vir.0.19456-0
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发表时间：
2004-02-01
影响因子：
3.8
通讯作者：
Sakae, K
中科院分区：
文献类型：
--
作者：
Ito, M;Yamashita, T;Sakae, K
从一名1岁短暂性麻痹患者的粪便标本中使用Vero细胞分离出一种细胞病变因子（A308/99）。该制剂直径约28 nm，具有明显的超微结构，类似于肠病毒的病毒颗粒。它不能被人肠道病毒、爱知病毒或人parechovirus等人小核糖核酸病毒的抗血清中和。该病毒粒子含有三种衣壳蛋白，分子量分别为38,30(。3和30kda。对A308/99的全核苷酸序列测定表明，该病毒的核苷酸和推导出的氨基酸序列与人parechovirus的核苷酸和氨基酸序列密切相关。对编码结构蛋白和非结构蛋白的11个区域进行比较，A308/99与人类1型parechovirus （HPeV-1）和2型parechovirus （HPeV-2）的核苷酸同源性分别为75% ~ 97%和73% ~ 97%。VP1与HPeV-1和HPeV-2分别具有74% -5%和73.1%的核苷酸同源性。与A308/99相关的病毒也从3例肠胃炎、湿疹或呼吸道疾病患者中分离出来。在HPeV-1和HPeV-2中，A308/99和其他3个分离株均不存在位于VP1 C端附近的精氨酸-甘氨酸-天冬氨酸基序。一项血清流行病学研究显示，A308/99抗体在婴儿中的患病率较低（15%），但随着年龄的增长而升高，到30岁时达到80%以上。这些观察结果表明，A308/99在遗传上接近HPeV-1和HPeV-2，但在血清学和遗传上不同，因此可归类为人类parechovirus的第三种血清型。
A cytopathic agent (A308/99) was isolated using Vero cells from a stool specimen of a 1-year-old patient with transient paralysis. The agent was approximately 28 nm in diameter with a distinct ultrastructure resembling the virus particle of an enterovirus. It could not be neutralized by antisera against human picornaviruses such as human enterovirus, Aichi virus or human parechovirus. The virion contained three capsid proteins with molecular masses of 38, 30(.)3 and 30 kDa. Determination of the complete nucleotide sequence of A308/99 revealed that the nucleotide and deduced amino acid sequences were closely related to those of human parechoviruses. When 11 regions encoding the structural and non-structural proteins were compared, A308/99 had between 75 and 97% and 73 and 97% nucleotide identity with human parechovirus type 1 (HPeV-1) and type 2 (HPeV-2), respectively. The most distinctive divergence was seen in VP1, which had 74-5% and 73.1% nucleotide identity with HPeV-1 and HPeV-2, respectively. Viruses related to A308/99 were also isolated from three patients with gastroenteritis, exanthema or respiratory illnesses. A308/99 and these other three isolates had no arginine-glycine-aspartic acid (RGD) motif, which is located near the C terminus of VP1 in HPeV-1 and HPeV-2. A seroepidemiological study revealed that the prevalence of A308/99 antibodies was low (15%) among infants but became higher with age, reaching more than 80% by 30 years of age. These observations indicate that A308/99 is genetically close to, but serologically and genetically distinct from, HPeV-1 and HPeV-2 and accordingly can be classified as third serotype of human parechovirus.