A new, quantitative, approach to the study of the stages of chemical cartinogenesis in the mouse's skin.
A new, quantitative, approach to the study of the stages of chemical cartinogenesis in the mouse's skin.
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DOI：
10.1038/bjc.1947.36
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发表时间：
1947-12
影响因子：
8.8
通讯作者：
SHUBIK, P
中科院分区：
文献类型：
--
作者：
BERENBLUM, I;SHUBIK, P
方法：使用实验室储备的混合品系小鼠（白色和有色），并在实验期间以适当的混合饲料饲养。用细剪刀定期修剪肩胛间区域的皮肤实验区域，以去除毛发，并用玻璃棒涂敷供试品溶液。如前一份来文（Berenblum和Shubik，1947年）所述，尽可能使用液体石蜡作为溶剂，以减少反应的变异性。正如Berenblum和Schoental（1947）所证明的那样，这就需要使用更高的浓度。
METHODS.Mice of mixed strain (white and coloured) from this laboratory stock were used, and were maintained throuighout the experiments on an adequate mixed diet. The experimental area of skin, in the inter-scapular region, was clipped periodically with fine scissors for the removal of hair, and the test solutions were applied with a glass rod. As in the previous communication (Berenblum and Shubik, 1947), liquid paraffin was, wherever possible, used as solvent, to diminish variability in response. This necessitated the use of higher concen-trations, as demonstratedby Berenblum and Schoental (1947).EXPERIMENTAL.