Relation between immunosuppressive PGE2 and IL-10 to pro-inflammatory IL-6 in seminal plasma of infertile and fertile men
Relation between immunosuppressive PGE2 and IL-10 to pro-inflammatory IL-6 in seminal plasma of infertile and fertile men
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DOI：
10.1080/01485010390129232
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发表时间：
2003-03-01
期刊：
影响因子：
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通讯作者：
Camejo, MI
中科院分区：
文献类型：
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作者：
Camejo, MI
体外研究表明，生育男性精浆比不育男性精浆具有更强的促有丝分裂抑制作用和更高的抗体依赖性细胞毒性抑制活性。精浆成分的变化是否可能解释这些结果？本研究旨在探讨不育和生育男性精浆中免疫抑制性PGE（2）和IL-10与促炎性IL-6的关系。应用特异性ELISA试剂盒检测了45例未确诊不育男性和25例生育男性精液中PGE（2）、IL-10和IL-6的含量。不育组精浆中IL-6浓度显著高于生育组（P <0.04）。然而，不育男性精液中的IL-10水平低于生育男性（p <0.04）。两组间PGE（2）浓度无差异。促炎性细胞因子如IL-6的增加和免疫抑制性细胞因子如IL-10的减少可改变男性和女性生殖道对精子细胞的耐受性，降低达到受精和着床的有利条件。有必要进行进一步的研究，包括其他促炎和抗炎物质，以评估精液免疫平衡在人类生育中的重要性。
In vitro studies have reported that seminal plasma of fertile men has more inhibitory mitogenic effect and higher suppressive activity on anti body-dependent cellular cytotoxicity than seminal plasma from infertile males. Is it possible that variations in the seminal plasma composition could explain these results? The present study was conducted to investigate the relation between immunosuppressive PGE(2) and IL-10 to pro-inflammatory IL-6 in seminal plasma of infertile and fertile men. Utilizing specific ELISA commercial kit, semen samples from 45 infertile undiagnosed and 25 fertile men were examined for the presence of PGE(2), IL-10, and IL-6. The IL-6 concentration in seminal plasma of infertile men was significantly higher than that of fertile men (p < .04). However, levels of IL-10 were lower for the semen of infertile men than that of fertile men (p < .04). No differences were observed in PGE(2) concentrations between both groups. Increase in pro-inflammatory cytokines, such as IL-6, and decrease in immunosuppressive ones, such as IL-10, could alter the tolerance to sperm cell in male and female tracts and reduce the favorable conditions to reach the fecundation and implantation. It is necessary to conduct further studies that include other pro- and anti-inflammatory substances to evaluate the importance of the immune balance of semen in human fertility.