A high-resolution comparative radiation hybrid map of equine chromosome 4q12-q22
A high-resolution comparative radiation hybrid map of equine chromosome 4q12-q22
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DOI：
10.1111/j.1365-2052.2006.01510.x
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发表时间：
2006-10-01
期刊：
影响因子：
2.4
通讯作者：
Distl, O.
中科院分区：
文献类型：
--
作者：
Dierks, C.;Momke, S.;Distl, O.
在这项研究中，我们提出了一个全面的5000拉德辐射杂交马4号染色体（ECA 4），包含马骨软骨病的数量性状位点的40厘米区域的地图。我们使用从马表达的序列标签或BAC克隆在ECA 4 q12-q22区域设计的引物，绘制了29个基因相关序列标签位点标记。在ECA 4 q12-q22区段中鉴定出三个保守的同线性区段，显示两个染色体断裂点。HSA 7 q的其他片段的标记定位于ECA 13 p和ECA 4p，HSA 7 p的一个区域与ECA 13 p同源。因此，我们已经提高了人-马比较图谱的分辨率，这使得能够识别感兴趣的潜在性状的候选基因。
In this study, we present a comprehensive 5000-rad radiation hybrid map of a 40-cM region on equine chromosome 4 (ECA4) that contains quantitative trait loci for equine osteochondrosis. We mapped 29 gene-associated sequence tagged site markers using primers designed from equine expressed sequence tags or BAC clones in the ECA4q12-q22 region. Three blocks of conserved synteny, showing two chromosomal breakpoints, were identified in the segment of ECA4q12-q22. Markers from other segments of HSA7q mapped to ECA13p and ECA4p, and a region of HSA7p was homologous to ECA13p. Therefore, we have improved the resolution of the human-equine comparative map, which allows the identification of candidate genes underlying traits of interest.