Comparison of two assays, a faecal egg count reduction test (FECRT) and a coproantigen reduction test (CRT), for the diagnosis of resistance to triclabendazole in Fasciola hepatica in sheep
Comparison of two assays, a faecal egg count reduction test (FECRT) and a coproantigen reduction test (CRT), for the diagnosis of resistance to triclabendazole in Fasciola hepatica in sheep
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DOI：
10.1016/j.vetpar.2010.10.057
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发表时间：
2011-03-10
影响因子：
2.6
通讯作者：
Fairweather, I.
中科院分区：
文献类型：
--
作者：
Flanagan, A.;Edgar, H. W. J.;Fairweather, I.
本试验采用粪卵计数减少试验（FECRT）和粪抗原减少试验（CRT）两种方法，对三氯苯达唑（TCBZ）对肝片吸虫耐药的诊断进行了评价。FECRT将成功的TCBZ治疗定义为治疗后14天（dpt）吸虫粪卵计数（FEC）减少95%或更多。CRT将有效的TCBZ治疗定义为在14 dpt时粪便对片形吸虫粪抗原呈阴性，如通过商业BIO K201粪抗原ELISA（Bio-X Diagnostics，Jemelle，Belgium）测量的。Hepatica分离株，先前分别描述为对TCBZ作用敏感（Cullompton和Fairhurst）和耐药（Leon和Oberon）。在感染后12周（wpi）对每个感染绵羊组中的一个亚组给予TCBZ治疗，并在治疗后4周（wpt）将这些绵羊剔除。在平行时间点（即16 wpi）剔除未给药绵羊亚组。进行尸检以确认治疗疗效。在整个试验期间，每周两次采集个体粪便样品，进行二次采样，并通过标准化的卵沉淀方案和BIO K201 ELISA进行检测。TCBZ。此外，该研究证实了Cullompton和Fairhurst F. hepatica分离株和Oberon F.肝分离物。然而，Leon F.发现肝菌分离物对TCBZ作用敏感，而不是耐药。(C)2010爱思唯尔有限公司版权所有。
A sheep trial was performed to evaluate two diagnostic assays, a faecal egg count reduction test (FECRT) and a coproantigen reduction test (CRT), for the diagnosis of resistance of Fasciola hepatica to triclabendazole (TCBZ). The FECRT defines successful TCBZ treatment as a 95% or greater reduction in fluke faecal egg counts (FECs) at 14 days post-treatment (dpt). The CRT defines effective TCBZ treatment as faeces negative for Fasciola coproantigens at 14 dpt, as measured by the commercial BIO K201 coproantigen ELISA (Bio-X Diagnostics, Jemelle, Belgium).Forty-nine indoor-reared sheep were split into four trial groups and each sheep was infected with 200 metacercariae of 1 of 4 F. hepatica isolates, previously described as susceptible (Cullompton and Fairhurst) and resistant (Leon and Oberon) to TCBZ action, respectively. TCBZ treatment was administered at 12 weeks post-infection (wpi) to one sub-group in each infected sheep group, and these sheep were culled at 4 weeks post-treatment (wpt). Untreated sheep sub-groups, were culled at a parallel time-point, that is, at 16 wpi. Necropsy was performed to confirm treatment efficacy. Individual faecal samples were collected twice-weekly throughout the trial period, sub-sampled and examined by a standardised egg sedimentation protocol and by the BIO K201 ELISA.Results supported the use of both the FECRT and the CRT for the diagnosis of resistance of F. hepatica to TCBZ. In addition, the study confirmed the TCBZ susceptibility of the Cullompton and Fairhurst F. hepatica isolates and the TCBZ resistance of the Oberon F. hepatica isolate. However, the Leon F. hepatica isolate was found to be susceptible, rather than resistant, to TCBZ action. (C) 2010 Elsevier B.V. All rights reserved.