The application of cell surface markers to demarcate distinct human pluripotent states
The application of cell surface markers to demarcate distinct human pluripotent states
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DOI：
10.1016/j.yexcr.2019.111749
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发表时间：
2020-02-01
影响因子：
3.7
通讯作者：
Rugg-Gunn, Peter J.
中科院分区：
文献类型：
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作者：
Goodwin, Jacob;Laslett, Andrew L.;Rugg-Gunn, Peter J.
人类多能干细胞（hPSC）研究的最新进展已经揭示了干细胞培养物中的不同亚群，并捕获了一系列具有不同分子和功能特性的多能状态。在多能性谱的两端是幼稚hPSC和致敏hPSC，由此在严格条件下生长的幼稚hPSC概括了植入前人胚胎的特征，并且常规生长的致敏hPSC更接近早期植入后胚胎排列。研究这些细胞类型将有助于确定控制早期发育的机制，并应提供对干细胞特性（如细胞身份，分化和重编程）的新见解。监测细胞表面标志物表达提供了一种有价值的方法来解析复杂的细胞群体，直接比较细胞类型，并分离活细胞进行功能实验。这篇综述讨论了细胞表面标记物的发现和应用，以研究人类多能细胞类型，特别关注幼稚和引发状态之间的转换。未来研究的重点领域包括所鉴定的细胞表面蛋白在多能性中的潜在功能、针对幼稚特异性蛋白表位的新的高质量单克隆抗体的生产以及使用细胞表面标记物在多能性状态中识别亚群。
Recent advances in human pluripotent stem cell (hPSC) research have uncovered different subpopulations within stem cell cultures and have captured a range of pluripotent states that hold distinct molecular and functional properties. At the two ends of the pluripotency spectrum are naive and primed hPSC, whereby naive hPSC grown in stringent conditions recapitulate features of the preimplantation human embryo, and the conventionally grown primed hPSC align closer to the early postimplantation embryo. Investigating these cell types will help to define the mechanisms that control early development and should provide new insights into stem cell properties such as cell identity, differentiation and reprogramming. Monitoring cell surface marker expression provides a valuable approach to resolve complex cell populations, to directly compare between cell types, and to isolate viable cells for functional experiments. This review discusses the discovery and applications of cell surface markers to study human pluripotent cell types with a particular focus on the transitions between naive and primed states. Highlighted areas for future study include the potential functions for the identified cell surface proteins in pluripotency, the production of new high-quality monoclonal antibodies to naive-specific protein epitopes and the use of cell surface markers to characterise subpopulations within pluripotent states.