Thermophoresis for characterizing biomolecular interaction
Thermophoresis for characterizing biomolecular interaction
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DOI：
10.1016/j.ymeth.2018.02.003
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发表时间：
2018-08-15
期刊：
影响因子：
4.8
通讯作者：
El Deeb, Sami
中科院分区：
文献类型：
--
作者：
Asmari, Mufarreh;Ratih, Ratih;El Deeb, Sami
生物分子相互作用的研究对于更深入地了解生物系统是至关重要的。蛋白质-蛋白质、蛋白质-核酸、蛋白质-糖、核酸-核酸和蛋白质小分子之间的相互作用支持着治疗和技术的发展。近年来，大量用于表征生物分子相互作用的分析技术的发展反映了这一领域极具前景的研究投资。近年来取得了很大进展，并建立了几个应用程序。MST是一种基于分子在微观温度梯度中的运动来定量结合事件的强大技术。与其他竞争技术相比，MST具有简单、自由的溶液分析、低样本量、分析时间短和固定化自由等优点。利用MST已经成功地开展了广泛的生物分子相互作用研究，这倾向于该技术的多功能性，用于筛选结合事件，以节省时间、成本和获得高数据质量。(C)2018 Elsevier Inc.保留所有权利。
The study of biomolecular interactions is crucial to get more insight into the biological system. The interactions of protein-protein, protein-nucleic acids, protein-sugars, nucleic acid-nucleic acids and protein small molecules are supporting therapeutics and technological developments. Recently, the development in a large number of analytical techniques for characterizing biomolecular interactions reflect the promising research investments in this field.In this review, microscale thermophoresis technology (MST) is presented as an analytical technique for characterizing biomolecular interactions. Recent years have seen much progress and several applications established. MST is a powerful technique in quantitation of binding events based on the movement of molecules in microscopic temperature gradient. Simplicity, free solutions analysis, low sample volume, short analysis time, and immobilization free are the MST advantages over other competitive techniques. A wide range of studies in biomolecular interactions have been successfully carried out using MST, which tend to the versatility of the technique to use in screening binding events in order to save time, cost and obtained high data quality. (C) 2018 Elsevier Inc. All rights reserved.