An integrated somatic cell hybrid, YAC, and BAC map of the Rmc1 region of mouse chromosome 1.
An integrated somatic cell hybrid, YAC, and BAC map of the Rmc1 region of mouse chromosome 1.
复制标题
小鼠 1 号染色体 Rmc1 区域的集成体细胞杂交、YAC 和 BAC 图谱。
DOI：
10.1006/geno.1999.5841
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发表时间：
1999
期刊：
影响因子：
--
通讯作者：
Housman,D
中科院分区：
文献类型：
--
作者：
Hunter,K;Greenwood,J;Yang,YL;Cunningham,JM;Birren,B;Housman,D
rmc 1是小鼠逆转录病毒多向性的细胞受体，决定了病毒的组织嗜性，因此在多向性病毒诱导的白血病的发病机制中起着关键作用。以前，我们报道了该基因的物理映射到小鼠1号染色体的5 cM区域，并在该区域构建了酵母人工染色体（YAC）重叠群。在这份报告中，我们描述的Rmc1候选区域的细化到约600 kb和一个集成的体细胞杂交，YAC，和跨越该地区的细菌人工染色体重叠群的产生。许多基因和基因座在物理上沿着染色体沿着，包括最近鉴定的Rmc 1候选基因。
Rmc1, the cellular receptor for the polytropic class of murine retroviruses, determines the tissue tropism of the virus and therefore plays a critical role in the pathogenesis of polytropic virus-induced leukemia. Previously we reported the physical mapping of this gene to a 5-cM region of mouse chromosome 1 and the construction of a yeast artificial chromosome (YAC) contig across this region. In this report we describe the refinement of the Rmc1 candidate region to approximately 600 kb and the generation of an integrated somatic cell hybrid, YAC, and bacterial artificial chromosome contig spanning the region. A number of genes and loci were physically ordered along the chromosome, including a recently identified candidate for Rmc1.