STUDIES OF IMMUNE FUNCTION OF CD-1 MICE EXPOSED TO AFLATOXIN-B1
STUDIES OF IMMUNE FUNCTION OF CD-1 MICE EXPOSED TO AFLATOXIN-B1
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DOI：
10.1016/0300-483x(87)90002-3
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发表时间：
1987-02-01
期刊：
影响因子：
4.5
通讯作者：
SHARMA, RP
中科院分区：
文献类型：
--
作者：
REDDY, RV;TAYLOR, MJ;SHARMA, RP
黄曲霉毒素B1（ABF 1）是黄曲霉的次级代谢产物，也是一种已知的动物肝癌原，在CD-1小鼠中评价了其免疫毒性潜力。雄性小鼠接受0，0.03，0.145或0.70毫克/公斤的黄曲霉毒素B1口服每隔一天2周的玉米油：乙醇车辆。脾淋巴细胞培养的存在下，脂多糖（LPS），植物血凝素（PHA），或美洲商陆有丝分裂原（PWM）。在淋巴细胞培养物中，有或没有上述促分裂剂，在暴露于AFB 1 2周后观察到剂量相关的[3 H]胸苷摄取抑制。在混合淋巴细胞培养中，DNA合成减少。用T依赖性抗原（绵羊红细胞）激发动物脾细胞产生的一级抗体受AFB 1影响。然而，当用T非依赖性抗原（LPS）攻击动物时，未观察到任何影响。观察到对匙孔血蓝蛋白的迟发型超敏反应的剂量相关抑制。结果表明，AFB_1对CD-1小鼠具有免疫毒性。
The immunotoxic potential of aflatoxin B1 (ABF1), a secondary metabolite of Aspergillus flavus and a known animal hepatocarcinogen, was evaluated in CD-1 mice. Male mice received 0, 0.03, 0.145 or 0.70 mg/kg of AFB1 orally every other day for 2 weeks in a corn oil:ethanol vehicle. Splenic lymphocytes were cultured in the presence of lipopolysaccharide (LPS), phytohemagglutinin (PHA), or pokeweed mitogen (PWM). A dose-related inhibition of [3H]thymidine uptake in lymphocyte cultures, with or without the above mitogens, was observed after 2 weeks of AFB1 exposure. Synthesis of DNA was decreased in mixed lymphocyte cultures. Primary antibody production by splenic cells, from animals challenged with a T-dependent antigen (sheep red blood cells), was affected by AFB1. No effects were observed, however, when animals were challenged with a T-independent antigen (LPS). A dose-related suppression of a delayed-type hypersensitivity reaction to keyhole limpet hemocyanin was observed. The results suggested that AFB1 was immunotoxic in CD-1 mice.