A simple isolation method for basal-lateral plasma membranes from rat kidney cortex.
A simple isolation method for basal-lateral plasma membranes from rat kidney cortex.
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一种从大鼠肾皮质中分离基底外侧质膜的简单方法。
DOI：
10.3109/09687688109065422
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发表时间：
1981
期刊：
影响因子：
--
通讯作者：
H. Murer
中科院分区：
文献类型：
--
作者：
V. Scalera;Yufei Huang;B. Hildmann;H. Murer
采用自取向Percoll(改良胶体二氧化硅)梯度从较重的微粒体膜组分中分离出(Na+-K+)-ATPase活性，经此过程可使(Na+-K+)-ATPase活性提高20倍。甲状旁腺素刺激腺苷环化酶活性6倍，精氨酸加压素、降钙素或异丙肾上腺素仅刺激1.5倍。基侧质膜的产量是匀浆中最初存在的量的5%到10%。该方法也适用于猪肾。
Basal-lateral membranes were separated in a self-orienting Percoll (modified colloidal silica) gradient from a heavy microsomal membrane fraction by centrifugation at 48,000g for 0.5 h. The (Na+--K+)-ATPase activity as a marker enzyme for the basal-lateral plasma membrane was 20-fold enriched by this procedure. The adenylate-cyclase activity measured in the basal-lateral membrane fraction was stimulated 6-fold by parathyrin and only up to 1.5-fold by arginine-vasopressin, calcitonin, or isoproterenol. The yield of basal-lateral plasma membranes was 5 to 10 percent of the amount initially present in the homogenate. The method is also applicable to the pig kidney.