Rapid detection of bacterial resistance to antibiotics using AFM cantilevers as nanomechanical sensors
Rapid detection of bacterial resistance to antibiotics using AFM cantilevers as nanomechanical sensors
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DOI：
10.1038/nnano.2013.120
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发表时间：
2013-07-01
影响因子：
38.3
通讯作者：
Kasas, S.
中科院分区：
文献类型：
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作者：
Longo, G.;Alonso-Sarduy, L.;Kasas, S.
药物的广泛滥用增加了多重耐药细菌的数量(1)，这意味着在感染管理中迫切需要能够快速检测和表征细菌对抗生素反应的工具。各种技术，如重天青还原试验(2)、分枝杆菌生长指示管(3)或基于聚合酶链式反应的方法(4)，已被用于研究细菌代谢及其对药物的反应。然而，许多细菌的价格相对较高，或者无法区分活的和死亡的细菌。在这里，我们展示了高灵敏的原子力显微镜悬臂的波动可以用于检测低浓度的细菌，表征它们的新陈代谢，并(在几分钟内)定量筛选它们对抗生素的反应。我们将这种方法应用于大肠杆菌和金黄色葡萄球菌，表明活细菌比接触抗生素的细菌产生更大的悬臂波动。我们的初步实验表明，这种波动与细菌新陈代谢有关。
The widespread misuse of drugs has increased the number of multiresistant bacteria(1), and this means that tools that can rapidly detect and characterize bacterial response to antibiotics are much needed in the management of infections. Various techniques, such as the resazurin-reduction assays(2), the mycobacterial growth indicator tube(3) or polymerase chain reaction-based methods(4), have been used to investigate bacterial metabolism and its response to drugs. However, many are relatively expensive or unable to distinguish between living and dead bacteria. Here we show that the fluctuations of highly sensitive atomic force microscope cantilevers can be used to detect low concentrations of bacteria, characterize their metabolism and quantitatively screen (within minutes) their response to antibiotics. We applied this methodology to Escherichia coli and Staphylococcus aureus, showing that live bacteria produced larger cantilever fluctuations than bacteria exposed to antibiotics. Our preliminary experiments suggest that the fluctuation is associated with bacterial metabolism.