Molecularly imprinted solid phase extraction using stable isotope labeled compounds as template and liquid chromatography–mass spectrometry for trace analysis of bisphenol A in water sample
Molecularly imprinted solid phase extraction using stable isotope labeled compounds as template and liquid chromatography–mass spectrometry for trace analysis of bisphenol A in water sample
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DOI：
10.1016/j.aca.2005.03.005
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发表时间：
2005-05
影响因子：
6.2
通讯作者：
Migaku Kawaguchi;Y. Hayatsu;H. Nakata;Y. Ishii;R. Ito;Koichi Saito;H. Nakazawa
中科院分区：
文献类型：
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作者：
Migaku Kawaguchi;Y. Hayatsu;H. Nakata;Y. Ishii;R. Ito;Koichi Saito;H. Nakazawa
我们以稳定同位素标记的化合物为模板分子，研制了一种分子印迹聚合物，称之为“同位素分子印迹聚合物”。在这项研究中，双酚A（BPA）被用作模型化合物。以双酚A（BPA）、4-叔丁基苯酚（BP）和双酚A-d16（BPA-d16）为模板分子，采用悬浮聚合法分别制备了无模板分子印迹聚合物（NIP）、MIP、虚拟分子印迹聚合物（DMIP）和IMIP。聚合物进行分子印迹固相萃取（MI-SPE），和提取的样品进行液相色谱-质谱（LC-MS）。虽然观察到BPA-d16从IMIP中泄漏，但未观察到BPA的泄漏。MIP和IMIP对BPA的选择性因子分别为4.45和4.43。因此，IMIP具有与MIP相同的分子识别能力。采用IMIP-MI-SPE-LC-MS联用技术分析河水样品，BPA的检出限为1 ppt，具有较高的灵敏度。此外，平均回收率高于99.8%（R.S.D.：3.7%），使用双酚A-13 C12（BPA-13 C12）作为替代标准。此外，还将IMIP应用于河水中BPA的MI-SPE液相色谱-质谱联用。河水样本中BPA的浓度被确定为32 pgml −1。我们证实，这是可能的，以测量痕量的目标分析物的MI-SPE使用IMIP。
We have developed a molecularly imprinted polymer (MIP) using a stable isotope labeled compound as the template molecule and called it the “isotope molecularly imprinted polymer” (IMIP). In this study, bisphenol A (BPA) was used as the model compound. None imprinted polymer (NIP), MIP, dummy molecularly imprinted polymer (DMIP) and IMIP were prepared by the suspension polymerization method using without template, BPA, 4-tert-butylphenol (BP) and bisphenol A-d16(BPA-d16), respectively. The polymers were subjected to molecularly imprinted solid phase extraction (MI-SPE), and the extracted samples were subjected to liquid chromatography–mass spectrometry (LC–MS). Although the leakage of BPA-d16from the IMIP was observed and that of BPA was not observed. The selectivity factors of MIP and IMIP for BPA were 4.45 and 4.43, respectively. Therefore, IMIP had the same molecular recognition ability as MIP. When MI-SPE with IMIP was used and followed by LC–MS in the analysis of river water sample, the detection limit of BPA was 1 ppt with high sensitivity. Moreover, the average recovery was higher than 99.8% (R.S.D.: 3.7%) by using bisphenol A-13C12(BPA-13C12) as the surrogate standard. In addition, the IMIP were employed in MI-SPE of BPA in river water sample by LC–MS. The concentration of BPA in the river water sample was determined to be 32pgml−1. We confirmed that it was possible to measure trace amounts of a target analyte by MI-SPE using IMIP.