Preparation of Chitosan/TPP Nanoparticles Used as Carrier in Enzyme Immobilization

Preparation of Chitosan/TPP Nanoparticles Used as Carrier in Enzyme Immobilization
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DOI:
10.4028/www.scientific.net/amr.718-720.271
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发表时间:
2013-07
期刊:
Advanced Materials Research
影响因子:
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通讯作者:
B. Sha;Qing-shan Liu;J. You;X. Ren;Hui Feng;X. Yin
B. Sha;Qing-shan Liu;J. You;X. Ren;Hui Feng;X. Yin
中科院分区:
其他
文献类型:
--
作者:
B. Sha;Qing-shan Liu;J. You;X. Ren;Hui Feng;X. Yin

文献摘要

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本文研究了壳聚糖纳米粒子作为固定化酶载体的制备方法。方法采用离子凝胶法制备纳米粒。采用响应面法对纳米粒的制备进行了研究。用扫描电镜和原子力显微镜对壳聚糖纳米粒的形貌进行了表征。结果采用适当的实验设计,考察了溶液pH值、壳聚糖浓度、组分配比等因素对壳聚糖/三磷酸盐(TPP)纳米粒制备的影响。
This work investigated the preparation of chitosan nanoparticles used as carriers for immobilized enzyme. Methods The nanoparticles were prepared by ionotropic gelation method. Response surface methodology was used in the preparation of nanoparticles. The morphologic characterization of chitosan nanoparticles was evaluated by scanning electron microscope and atomic force microscope. Results Using an appropriate experimental design, we have studied the significant influence factors (pH of the solution, concentrations of the chitosan , ratios of components) in the preparation of chitosan/triphosphate (TPP) nanoparticles.