Lamivudine Inhibits the Replication of ALV-J Associated Acutely Transforming Virus and its Helper Virus and Tumor Growth In vitro and In vivo.
Lamivudine Inhibits the Replication of ALV-J Associated Acutely Transforming Virus and its Helper Virus and Tumor Growth In vitro and In vivo.
复制标题
拉米夫定在体外和体内抑制ALV-J相关急性转化病毒及其辅助病毒的复制和肿瘤生长
DOI：
10.3389/fmicb.2015.01306
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发表时间：
2015
影响因子：
5.2
通讯作者：
Cui Z
中科院分区：
文献类型：
--
作者：
Wang Y;Xu S;Li S;Su H;Chang S;Li Y;Sun X;Zhao P;Cui Z
为研究拉米夫定对禽白血病病毒J亚群(ALV-J)的抗病毒作用及其对急性转化禽白血病病毒(ALV-J)所致纤维肉瘤生长的抑制作用，在鸡胚成纤维细胞培养和1日龄雏鸡接种急性转化病毒株(SDAU1005)上进行了一系列实验。该疫苗取自养鸡场的一例急性纤维肉瘤，含有携带v-fps癌基因的复制缺陷病毒Fu-J及其辅助病毒ALV-J株SDAU1005。细胞培养的三种不同检测结果表明，拉米夫定对SDAU1005和FU-J病毒的复制均有明显的抑制作用。在鸡试验中，拉米夫定能降低雏鸡血浆中SDAU1005和FU-J的病毒载量，延缓急性肉瘤的出现，并能降低雏鸡早期死亡率。在鸡试验中，拉米夫定能降低雏鸡血浆中SDAU1005和FU-J的病毒载量，延缓急性肉瘤的出现，降低雏鸡的早期死亡率。此模型可用于直接评估拉米夫定对此类肿瘤的抑制作用，并在评估肿瘤过程的同时了解复制缺陷病毒及其辅助病毒之间的关系。
To study the antiviral effects of lamivudine on avian leukosis virus subgroup J (ALV-J) and its inhibitory effect on the growth of fibrosarcomas caused by acute transforming avian leukosis virus, a series of experiments were performed in chicken embryo fibroblast cultures and 1-day-old chickens inoculated with an acutely transforming viral stock Fu-J (SDAU1005). This stock was prepared from an acutely fibrosarcoma of field cases in chicken farms and contained both the replication-defective virus Fu-J carrying v-fps oncogene and its helper virus ALV-J strain SDAU1005. The results from three different assays in cell cultures demonstrated the significant inhibitory effect of lamivudine on the replication of both SDAU1005 and Fu-J viruses. Furthermore, the effect was dose dependent in the concentration range of 1–4 μg/ml. In chicken experiments, lamivudine could decrease the viral loads of SDAU1005 and Fu-J in the plasma of inoculated chickens, delay the appearance of acute sarcomas, and decrease chicken mortality in the early stage. This model may be used to directly evaluate the inhibitory effects of lamivudine on such tumors and to understand the relationship between the replication-defective virus and its helper virus while also assessing tumor processes.