CELLULAR STRATEGIES TO PROMOTE VASCULARISATION IN TISSUE ENGINEERING APPLICATIONS
CELLULAR STRATEGIES TO PROMOTE VASCULARISATION IN TISSUE ENGINEERING APPLICATIONS
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DOI：
10.22203/ecm.v028a05
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发表时间：
2014-07-01
影响因子：
3.1
通讯作者：
Guerreiro, S. G.
中科院分区：
文献类型：
--
作者：
Costa-Almeida, R.;Granja, P. L.;Guerreiro, S. G.
血管化被认为是组织工程中最大的挑战之一。存在不同的策略，但基于细胞的方法已成为一种有前途的治疗方法，以实现成功的血管化。使用内皮细胞来工程化血管化组织已被广泛研究。这一研究领域随着内皮祖细胞的发现而发展，内皮祖细胞是一种具有高再生潜力的亚群。然而，单独的内皮细胞群体的存活似乎受到损害。为了克服这个问题，已经开发了涉及支持细胞（如壁细胞、成纤维细胞或更多组织特异性细胞）的共培养系统。内皮细胞受益于支持细胞产生的细胞外基质组分和生长因子，这导致新血管稳定和成熟。在共培养系统中使用内皮祖细胞似乎是在日益复杂的方法中促进血管形成的有前景的策略。在本文中，作者提供了一个细胞策略的概述，可用于增加组织工程和再生中的血管化。
Vascularisation is considered to be one of the greatest challenges in tissue engineering. Different strategies exist but cell-based approaches have emerged as a promising therapy to achieve successful vascularisation. The use of endothelial cells to engineer vascularised tissues has been extensively investigated. This field of research has evolved with the discovery of endothelial progenitor cells, a subpopulation with a high regenerative potential. However, the survival of endothelial cell populations alone seems to be impaired. To overcome this problem, co-culture systems, involving supporting cells, like mural cells, fibroblasts, or more tissue-specific cells have been developed. Endothelial cells benefit from the extracellular matrix components and growth factors produced by the supporting cells, which results in neovessel stabilisation and maturation. The use of endothelial progenitor cells in co-culture systems appears to be a promising strategy to promote vascularisation in approaches of increasing complexity. Herein, the authors provide an overview of the cellular strategies that can be used for increasing vascularisation in tissue engineering and regeneration.