Isolation and Characterization of Six Abscisic Acid-Inducible Genes from Carrot Somatic Embryos
Isolation and Characterization of Six Abscisic Acid-Inducible Genes from Carrot Somatic Embryos
复制标题
DOI：
10.5511/plantbiotechnology.21.309
复制
发表时间：
2004-12
影响因子：
1.6
通讯作者：
H. Shiota;Guangxiao Yang;S. Shen;Chang-Ho Eun;K. Watabe;I. Tanaka;H. Kamada
中科院分区：
文献类型：
--
作者：
H. Shiota;Guangxiao Yang;S. Shen;Chang-Ho Eun;K. Watabe;I. Tanaka;H. Kamada
In carrot (Daucus carota L.) somatic embryos, desiccation tolerance is induced by treatment with abscisic acid (ABA). Six cDNA clones that showed ABA-enhanced expression were isolated from carrot by differential screening with ABA-treated and ABA-untreated somatic embryos, and they were named the CAISE (carrot ABA-induced in somatic embryos) genes. Five of the clones encode late embryogenesis abundant (LEA) proteins, and the other clone encodes a glucose and ribitol dehydrogenase. The expression of the CAISE genes was detected in maturing seeds, embryogenic cells, and ABA-treated somatic embryos in which exhibit desiccation tolerance induced by endogenous or exogenous ABA. These results indicate that ABA-induced desiccation tolerance in carrot somatic embryos may be induced by the LEA proteins and the glucose and ribitol dehydrogenase encoded by these ABA-inducible genes.
