Progenitor cells of the olfactory receptor neuron lineage
Progenitor cells of the olfactory receptor neuron lineage
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DOI：
10.1002/jemt.10147
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发表时间：
2002-08-01
影响因子：
2.5
通讯作者：
Wu, HH
中科院分区：
文献类型：
--
作者：
Calof, AL;Bonnin, A;Wu, HH
小鼠嗅上皮具有许多特性，使其成为研究神经发生分子调控的理想系统。我们已经使用了体外和体内的方法相结合，以确定三个不同阶段的嗅觉受体神经元谱系的神经元祖细胞。神经元干细胞最终负责该系统中的连续神经元更新，产生通过其转录因子MASHL的表达鉴定的转运放大祖细胞。表达MASH1的祖细胞产生第二个转运放大祖细胞，即直接神经元前体，其与干细胞和表达MASH1的祖细胞不同，并定量产生嗅觉受体神经元。祖细胞增殖和分化的调节发生在这三个细胞阶段中的每一个阶段，并且成纤维细胞生长因子（FGF）和骨形态发生蛋白（BMP）家族的生长因子似乎在这些过程中发挥特别重要的作用。FGF和BMP的作用分析表明，在嗅觉神经发生的调节中，负信号至少起着与正信号同样重要的作用。
The olfactory epithelium of the mouse has many properties that make it an ideal system for studying the molecular regulation of neurogenesis. We have used a combination of in vitro and in vivo approaches to identify three distinct stages of neuronal progenitors in the olfactory receptor neuron lineage. The neuronal stem cell, which is ultimately responsible for continual neuron renewal in this system, gives rise to a transit amplifying progenitor identified by its expression of a transcription factor, MASHL The MASH1-expressing progenitor gives rise to a second transit amplifying progenitor, the Immediate Neuronal Precursor, which is distinct from the stem cell and MASH1-expressing progenitor, and gives rise quantitatively to olfactory receptor neurons. Regulation of progenitor cell proliferation and differentiation occurs at each of these three cell stages, and growth factors of the fibroblast growth factor (FGF) and bone morphogenetic protein (BMP) families appear to play particularly important roles in these processes. Analyses of the actions of FGFs and BMPs reveal that negative signaling plays at least as important a role as positive signaling in the regulation of olfactory neurogenesis.