Gestational changes in production of NO and expression of NOS mRNA isoforms in the rat placenta.
Gestational changes in production of NO and expression of NOS mRNA isoforms in the rat placenta.
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DOI：
10.1292/jvms.71.495
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发表时间：
2009-04
期刊：
影响因子：
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通讯作者：
Takehito Suzuki;Y. Ikeda;H. Yoshikawa;Kazuaki Tanaka;H. Morita;Masako Yamamoto;T. Takizawa
中科院分区：
文献类型：
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作者：
Takehito Suzuki;Y. Ikeda;H. Yoshikawa;Kazuaki Tanaka;H. Morita;Masako Yamamoto;T. Takizawa
为了监测胎盘NO生成量随时间的变化，我们使用Fe-N-(二硫代羧基)肌氨酸(Fe-DTCS)络合物作为NO捕捉剂的电子顺磁共振波谱。定量RT-PCR法平行检测一氧化氮合酶(NOS)亚型的表达。在妊娠13.5天首次检测不到任何产物。NO水平在15.5天达到高峰，之后在妊娠的最后几天显著下降。NOSIImRNA的表达模式与NO水平的变化具有良好的一致性，而NOSIIImRNA的表达在妊娠过程中变化不明显。因此，胎盘中的NO水平似乎是一氧化氮合酶II依赖的，并且在不同的妊娠阶段有所不同。
To monitor changes in NO production over time in the fetal placenta of rats, we used electron paramagnetic resonance spectroscopy with the Fe-N-(dithiocarboxy) sarcosine (Fe-DTCS) complex as an NO-trapping reagent. The expression of nitric oxide synthase (NOS) isoforms was examined in parallel using quantitative RT-PCR. NO production was first detected on day 13.5 of gestation. NO levels reached a peak on day 15.5, then decreased significantly during the last few days of gestation. The pattern of expression of NOS II mRNA was in good agreement with changes in NO levels, whereas NOS III mRNA expression did not change markedly during gestation. Thus, it appears that NO levels in the placenta are NOS II-dependent and differ at different gestational stages.