Drosophila klarsicht has distinct subcellular localization domains for nuclear envelope and microtubule localization in the eye
Drosophila klarsicht has distinct subcellular localization domains for nuclear envelope and microtubule localization in the eye
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DOI：
10.1534/genetics.104.028662
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发表时间：
2004-11-01
期刊：
影响因子：
3.3
通讯作者：
Hook, J
中科院分区：
文献类型：
--
作者：
Fischer, JA;Acosta, S;Hook, J
果蝇klarsicht（klar）基因是眼睛中感光细胞核发育调节迁移所必需的。klar编码一个分子量约为250 kD的大蛋白，其C端只有一个可识别的氨基酸序列基序，即KASH（Klar，Anc-1，Syne-1 homology）结构域。有人提出Klar通过将细胞核连接到微管组织中心（MTOC）来促进细胞核迁移。在这里，我们进行遗传和免疫组化实验，提供了一个关键的测试这个模型。我们分析了内源性klar基因的突变体，以及从转基因中表达缺失形式的Klar蛋白的果蝇。我们发现，KASH结构域的Klar是至关重要的核周定位和功能。此外，我们发现，N-末端部分的Klar也是重要的功能，并包含一个域，定位的蛋白质微管顶端的细胞核。这些结果提供了强有力的支持，在该模型中，Klar链接的核MTOC。
The Drosophila klarsicht (klar) gene is required for developmentally regulated migrations of photoreceptor cell nuclei in the eye. klar encodes a large (similar to250 kD) protein with only one recognizable amino acid sequence motif, a KASH (Klar, Anc-1, Syne-1 homology) domain, at its C terminus. It has been proposed that Klar facilitates nuclear migration by linking the nucleus to the microtubule organizing center (MTOC). Here we perform genetic and immunohistochemical experiments that provide a critical test of this model. We analyze mutants in the endogenous klar gene and also flies that express deleted forms of Klar protein from transgenes. We find that the KASH domain of Klar is critical for perinuclear localization and for function. In addition, we find that the N-terminal portion of Klar is also important for function and contains a domain that localizes the protein to microtubules apical to the nucleus. These results provide strong support for a model in which Klar links the nucleus to the MTOC.