Lower doses of vanadate in combination with trigonella restore altered carbohydrate metabolism and antioxidant status in alloxan-diabetic rats
Lower doses of vanadate in combination with trigonella restore altered carbohydrate metabolism and antioxidant status in alloxan-diabetic rats
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DOI：
10.1016/j.cccn.2003.12.005
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发表时间：
2004-04-01
影响因子：
5
通讯作者：
Baquer, NZ
中科院分区：
文献类型：
--
作者：
Mohammad, S;Taha, A;Baquer, NZ
背景：钒酸盐治疗糖尿病大鼠已被报道纠正改变的碳水化合物代谢和抗氧化状态。然而，钒酸盐在相对高的剂量下发挥这些作用，并产生几种毒性作用。我们使用低剂量的钒酸盐与胡芦巴种子粉（TSP）相结合，并评估其对糖尿病大鼠酶变化的影响。研究方法：四氧嘧啶糖尿病大鼠分别用胰岛素、钒酸盐（0.6mg/ml）、TSP以及钒酸盐和TSP联合给药21天。在实验期结束时，血糖水平和活性的丙酮酸激酶（PK），磷酸烯醇式丙酮酸羧激酶（PEPCK），谷胱甘肽过氧化物酶（GPx），谷胱甘肽还原酶（GR），超氧化物歧化酶（SOD）和过氧化氢酶（CAT）在肝和肾的胞浆级分进行了测量。结果：糖尿病大鼠血糖明显升高。用抗糖尿病化合物治疗导致葡萄糖水平降低。用钒酸盐和胡芦巴联合剂量处理的大鼠的葡萄糖水平与对照组相当。糖尿病大鼠肝、肾组织中PK、PEPCK、SOD、GPx、GR、CAT活性也有类似的变化。钒酸盐和胡芦巴的组合剂量被认为是最有效的纠正这些变化。结论：较低剂量的钒酸盐可与TSP联合使用，以有效地对抗糖尿病的变化，没有任何毒副作用。(C)2004 Elsevier B. V.保留所有权利。
Background: Vanadate treatment to diabetic rats has been reported to correct the altered carbohydrate metabolism and antioxidant status. However, vanadate exerts these effects at relatively high doses and several toxic effects are produced. We used low doses of vanadate in combination with Trigonella foenum graecum seed powder (TSP) and evaluated their effect on the enzyme changes in diabetic rats. Methods: Alloxan-diabetic rats were treated separately with insulin, vanadate (0.6 mg/ml), TSP and a combined dose of Vanadate (0.2 mg/ml) and TSP for 21 days. At the end of the experimental period, blood glucose levels and activities of pyruvate kinase (PK), phosphoenolpyruvate carboxykinase (PEPCK), glutathione peroxidase (GPx), glutathione reductase (GR), superoxide dismutase (SOD) and catalase (CAT) were measured in cytosolic fraction in the liver and kidney. Results: Blood glucose levels increased markedly in diabetic rats. Treatment with antidiabetic compounds resulted in the reduction of glucose levels. Rats treated with combined dose of vanadate and trigonella had glucose levels comparable to control ones. Similar results were obtained with the activities of PK, PEPCK, SOD, GPx, GR, and CAT in liver and kidney of diabetic rats. Combined dose of vanadate and Trigonella was found to be most effective in correcting these alterations. Conclusions: Lower doses of vanadate could be used in combination with TSP to effectively counter diabetic alterations without any toxic side effects. (C) 2004 Elsevier B.V. All rights reserved.