Transcriptional and posttranscriptional regulation of Shigella shuT in response to host-associated iron availability and temperature.
Transcriptional and posttranscriptional regulation of Shigella shuT in response to host-associated iron availability and temperature.
复制标题
志贺氏菌 shuT 的转录和转录后调节响应宿主相关的铁可用性和温度。
DOI：
10.1002/mbo3.442
复制
发表时间：
2017
期刊：
影响因子：
3.4
通讯作者：
Murphy,ErinR
中科院分区：
文献类型：
--
作者：
Wei,Yahan;Kouse,AndrewB;Murphy,ErinR
Like most bacteria,Shigellamust maintain a precise balance between the necessity and toxicity of iron; a balance that is achieved, at least in part, by regulating the production of bacterial iron acquisition systems in response to specific environmental signals. Using theShigellaheme utilization (Shu) system,S. dysenteriaeis able to acquire iron from heme, a potentially rich source of nutritional iron within the otherwise iron‐limited environment of the human host. Investigations presented within reveal two distinct molecular mechanisms underlying previously uncharacterized transcriptional and translational regulation ofshuT, a gene encoding the periplasmic‐binding component of the Shu system. WhileshuTtranscription is regulated in response to iron availability via a process dependent upon the global regulator Fur and a Fur‐binding site located immediately downstream of the promoter,shuTtranslation is regulated in response to environmental temperature via the activity of an RNA thermometer located within the 5′ untranslated region of the gene. Such complex regulation likely increases the fitness ofS. dysenteriaeby ensuring maximal ShuT production when the pathogen is within the iron‐limited and relatively warm environment of the infected host, the only environment in which heme will be encountered as a potential source of essential iron.
