Sindbis virus glycoproteins: effect of the host cell on the oligosaccharides
Sindbis virus glycoproteins: effect of the host cell on the oligosaccharides
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辛德毕斯病毒糖蛋白：宿主细胞对寡糖的影响
DOI：
10.1128/jvi.16.3.613-620.1975
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发表时间：
1975
影响因子：
5.4
通讯作者：
D. Burke
中科院分区：
文献类型：
--
作者：
K. Keegstra;B. Sefton;D. Burke
辛德比斯病毒在四种不同的宿主细胞中生长，并分析了糖蛋白的碳水化合物部分。在 BHK-21 细胞中生长的辛德比斯病毒比在鸡胚细胞中生长的辛德比斯病毒含有更多的唾液酸和半乳糖。在其他方面，这两种宿主中生长的病毒的碳水化合物非常相似。还检测了在劳斯肉瘤病毒转化的鸡细胞中或在多瘤病毒转化的 BHK 细胞中生长的辛德比斯病毒。比较来自正常细胞和转化细胞的病毒，观察到唾液酸含量的差异；但除此之外，碳水化合物的结构看起来基本相似。宿主细胞的生长条件也影响病毒糖蛋白碳水化合物链的完成程度。
Sindbis virus was grown in four different host cells and the carbohydrate portions of the glycoproteins were analyzed. Sindbis virus grown in BHK-21 cells has more sialic acid and galactose than Sindbis virus grown in chicken embryo cells. In other respects the carbohydrates from virus grown in these two hosts are very similar. Sindbis virus grown either in chick cells transformed by Rous sarcoma virus or in BHK cells transformed by polyoma virus was also examined. In comparisons of virus from normal and transformed cells, differences in the amount of sialic acid were observed; but otherwise the carbohydrate structures appeared basically similar. The growth conditions used for the host cell also affected the degree of completion of the carbohydrate chains of the viral glycoproteins.