The Arabidopsis COMATOSE locus regulates germination potential.
The Arabidopsis COMATOSE locus regulates germination potential.
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发表时间：
2000-09
期刊：
影响因子：
4.6
通讯作者：
Laurel Russell;Victoria S. Larner;Smita Kurup;Susan Bougourd;Michael J. Holdsworth
中科院分区：
文献类型：
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作者：
Laurel Russell;Victoria S. Larner;Smita Kurup;Susan Bougourd;Michael J. Holdsworth
拟南芥中COMATOSE基因座的突变导致发芽潜力显著降低。虽然昏迷（cts）胚的形态没有改变，但生理分析表明成熟的cts种子对赤霉素没有反应。长时间的低温只能部分恢复吸胀种子的发芽能力，种子成熟后不能恢复。遗传分析表明，cts表型在胚中表达，在植物生长发育的其他阶段没有观察到野生型和突变体植物之间的表型差异。因此，cts代表了一类新的突变体，具有导致发芽能力严重受损的特定病变。分析了cts和调控胚胎成熟的基因座之间的遗传相互作用，以及脱落酸的生物合成和感知。从这些研究结果表明，cts突变表型需要这些位点的野生型的行动，并建议，CTS对这些位点发挥抑制功能。一个模型提出假设，CTS促进发芽势增加，抑制胚休眠。CTS的这些功能可能导致胚休眠的解除，这是萌发的先决条件。
Mutation of the COMATOSE locus in Arabidopsis results in a marked reduction in germination potential. Whilst the morphology of comatose (cts) embryos is not altered, physiological analysis reveals that mature cts seeds do not respond to gibberellin. Prolonged chilling of imbibed seeds only partially restores germination potential, and seeds do not after ripen. Genetic analysis shows that the cts phenotype is expressed in the embryo and phenotypic differences between wild-type and mutant plants were not observed during other stages of plant growth and development. Therefore cts represents a new class of mutant, with a specific lesion that results in severely impaired germination potential. Genetic interactions were analysed between cts and loci that regulate embryo maturation, and abscisic acid biosynthesis and perception. Results from these studies showed that the cts mutant phenotype required the wild-type action of these loci, and suggested that CTS exerts a repressive function on these loci. A model is presented postulating that CTS promotes increased germination potential, and represses embryo dormancy. These functions of CTS may result in the removal of embryo dormancy as a prerequisite to germination.