Evaluation of the efficacy of electrochemically activated solutions against nosocomial pathogens and bacterial endospores
Evaluation of the efficacy of electrochemically activated solutions against nosocomial pathogens and bacterial endospores
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DOI：
10.1111/j.1472-765x.2009.02790.x
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发表时间：
2010-03-01
影响因子：
2.4
通讯作者：
Reynolds, D. M.
中科院分区：
文献类型：
--
作者：
Robinson, G. M.;Lee, S. W-H.;Reynolds, D. M.
目的：电化学活化溶液（ECAS）是通过专门设计的电解池从卤化物盐溶液中产生的。已知活性溶液对广泛的目标微生物物种具有高杀生物活性，然而，揭示这些溶液的生物降解动力学的文献有限。该研究的目的是确定的ECAS的目标物种（包括内生孢子）的范围内使用的ECAS的种群杀灭率和程度在阳极（anolyte.Methods和结果：耐甲氧西林金黄色葡萄球菌，铜绿假单胞菌，萎缩芽孢杆菌孢子和艰难梭菌孢子的标准悬浮液进行了处理与阳极电解液在定量悬浮液测定。对于营养细胞，测试的所有阳极电解液浓度在10 s内将活菌群降低至检测限以下。在99%的浓度下，阳极电解液在活B中产生大于5的log（10）减少因子。90s内孢子萎缩，C.在浓度为5%或更高时，在20 s内将艰难杆菌内生孢子降至实验检测限以下。结论：阳极电解液在杀灭试验细菌和孢子方面非常有效。在低至1%的稀释度下对营养细胞和对C.研究的意义和影响：本研究的结果表明，ECAS在较低浓度下有效，并且比以前报道的作用更迅速。有效的杀菌和杀孢子活性加上使用点生成，低生产成本和环境相容性表明，酸性ECAS有可能成为当前消毒剂库的有用补充。
Aims:Electrochemically activated solutions (ECAS) are generated from halide salt solutions via specially designed electrolytic cells. The active solutions are known to possess high biocidal activity against a wide range of target microbial species, however, literature revealing the kill-kinetics of these solutions is limited. The aim of the study was to identify the kill-rate and extent of population kill for a range of target species (including endospores) using ECAS generated at the anode (anolyte).Methods and Results:Standard suspensions of methicillin-resistant Staphylococcus aureus, Pseudomonas aeruginosa, Bacillus atrophaeus spores and Clostridium difficile spores were treated with anolyte in a quantitative suspension assay. For vegetative cells, all concentrations of anolyte tested reduced the viable population to below the detection limit within 10 s. At a concentration of 99%, anolyte produced a log(10) reduction factor of greater than five in viable B. atrophaeus endospores within 90 s and reduced numbers of C. difficile endospores to below the experimental detection limit within 20 s at concentrations of 5% or greater.Conclusions:Anolyte was highly effective in killing test-bacteria and spores. The bactericidal efficacy was retained against vegetative cells at dilutions as low as 1% and against C. difficile spores as low as 5%.Significance and Impact of Study:The results of this study demonstrate that ECAS are effective at lower concentrations and act more rapidly than previously reported. Potent bactericidal and sporicidal activity coupled with point-of-use generation, low production-costs and environmental compatibility suggest that acidic ECAS has the potential to be a useful addition to the current armoury of disinfectants.