Molecular and immunohistochemical detection of rotavirus in urinary sediment cells of children with rotavirus gastroenteritis
Molecular and immunohistochemical detection of rotavirus in urinary sediment cells of children with rotavirus gastroenteritis
复制标题
DOI：
10.1111/j.1469-0691.2011.03522.x
复制
发表时间：
2011-08-01
影响因子：
14.2
通讯作者：
Yachie, A.
中科院分区：
文献类型：
--
作者：
Yokoyama, T.;Sugimoto, N.;Yachie, A.
这是第一份显示轮状病毒感染免疫功能正常的轮状病毒胃肠炎儿童的尿沉渣细胞的报告。我们发现轮状病毒胃肠炎患者的尿液样本中经常检测到含有包涵体的细胞。在我们的免疫组织化学分析中，这些细胞呈细胞角蛋白阳性，有时与轮状病毒抗原共表达。此外，在巢式RT-PCR实验中，我们在轮状病毒胃肠炎患者的一些尿液样本中检测到了轮状病毒双链RNA。我们的结论是，轮状病毒可能导致尿沉渣细胞感染，并伴有轮状病毒胃肠炎。
This is the first report showing that rotavirus infects the urinary sediment cells in immunocompetent children with rotavirus gastroenteritis. We found that inclusion-bearing cells were frequently detected in the urine samples of patients with rotavirus gastroenteritis. These cells were positive for cytokeratin, which was sometimes coexpressed with rotavirus antigen, in our immunohistochemical analysis. Moreover, in nested RT-PCR experiments, we detected rotavirus double-stranded RNA in some urine samples of patients with rotavirus gastroenteritis. We concluded that rotavirus could lead to infection of the urinary sediment cells concomitantly with rotavirus gastroenteritis.