Nile Red nucleoside: novel nucleoside analog with a fluorophore replacing the DNA base.
Nile Red nucleoside: novel nucleoside analog with a fluorophore replacing the DNA base.
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DOI：
10.1093/nass/49.1.155
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发表时间：
2005-09
期刊：
影响因子：
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通讯作者：
Kazuki Tainaka;Yoshimasa Fujiwara;A. Okamoto
中科院分区：
文献类型：
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作者：
Kazuki Tainaka;Yoshimasa Fujiwara;A. Okamoto
溶致变色染料在研究生物体系中的局部极性和动力学方面非常有用。合成了一种新型的溶致变色发色团尼罗红-C-2‘-脱氧核糖苷，作为光物理探针，并选择性地掺入到寡核苷酸中。尼罗红核苷的吸收光谱和荧光光谱对溶剂的极性有明显的敏感性。
A solvatochromic dye is very useful in studies of local polarity and dynamics in biological systems. A novel solvatochromic chromophore, Nile Red beta-C-2'-deoxyriboside, has been synthesized to act as a photophysical probe, and incorporated site-selectively into an oligodeoxynucleotide. The absorption and fluorescence spectra of Nile Red nucleoside showed a remarkable sensitivity to the solvent polarity.