Seeing is believing? A beginners' guide to practical pitfalls in image acquisition
Seeing is believing? A beginners' guide to practical pitfalls in image acquisition
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DOI：
10.1083/jcb.200507103
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发表时间：
2006-01-02
影响因子：
7.8
通讯作者：
North, AJ
中科院分区：
文献类型：
--
作者：
North, AJ
成像可以被认为是最直接的实验。你看到了什么;你报告你所看到的。如果事情真的这么简单就好了。现代成像技术已经给我们可以处理的问题带来了革命，但这是以越来越复杂的设备为代价的。此外，在试图市场竞争的系统，显微镜往往被不恰当地描述为易于使用，适合初学者。对实验操作和设备设置的理解不足导致图像采集过程中引入错误。在这篇文章中，我回顾了研究人员在图像采集过程中面临的一些最常见的实际陷阱，以及它们如何影响实验数据的解释。这篇文章既不是针对推动成像技术前沿的显微镜大师，也不是针对我的成像专家同事，他们可能会对过于简单的评论和缺乏细节感到畏缩。相反，这是为初学者准备的，当他们听到“共聚焦针孔”这个词时，他们会惊恐地吞咽，或者当他们在显微镜下一次又一次地看着他们的细胞在他们眼前褪色和死亡时，他们会叹息。新手们，振作起来，如果显微镜真的这么简单，那么很多人（包括我自己）都会突然失业！
Imaging can be thought of as the most direct of experiments. You see something; you report what you see. If only things were truly this simple. Modern imaging technology has brought about a revolution in the kinds of questions we can approach, but this comes at the price of increasingly complex equipment. Moreover, in an attempt to market competing systems, the microscopes have often been inappropriately described as easy to use and suitable for near-beginners. Insufficient understanding of the experimental manipulations and equipment set-up leads to the introduction of errors during image acquisition. In this feature, I review some of the most common practical pitfalls faced by researchers during image acquisition, and how they can affect the interpretation of the experimental data.This article is targeted neither to the microscopy gurus who push forward the frontiers of imaging technology nor to my imaging specialist colleagues who may wince at the overly simplistic comments and lack of detail. Instead, this is for beginners who gulp with alarm when they hear the word “confocal pinhole” or sigh as they watch their cells fade and die in front of their very eyes time and time again at the microscope. Take heart, beginners, if microscopes were actually so simple then many people (including myself) would suddenly be out of a job!