VARIATIONS IN CELLULAR PROLIFERATION AND MATRIX SYNTHESIS IN INTRASYNOVIAL AND EXTRASYNOVIAL TENDONS - AN IN-VITRO STUDY IN DOGS
VARIATIONS IN CELLULAR PROLIFERATION AND MATRIX SYNTHESIS IN INTRASYNOVIAL AND EXTRASYNOVIAL TENDONS - AN IN-VITRO STUDY IN DOGS
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DOI：
10.1016/0363-5023(94)90016-7
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发表时间：
1994-03-01
影响因子：
1.9
通讯作者：
LOHMANDER, SL
中科院分区：
文献类型：
--
作者：
ABRAHAMSSON, SO;GELBERMAN, RH;LOHMANDER, SL
最近显示，滑膜内和滑膜外屈肌腱移植物在指鞘内的细胞存活和宿主整合模式不同。为了确定这两种类型的肌腱之间是否存在根本差异，我们研究了在 MCDB 105 和 DMEM 培养基中进行短期外植体培养的 12 只成年杂种狗的滑膜内和滑膜外肌腱节段的生化成分和细胞活性。在两种培养基中培养后测定蛋白聚糖、胶原蛋白和非胶原蛋白的合成和含量以及DNA合成。与滑膜外肌腱相比，在 MCDB 105 培养基中培养的滑膜内肌腱片段合成的胶原蛋白、非胶原蛋白和 DNA 显着减少，并且具有相似数量的蛋白聚糖。 DMEM 培养基中滑膜内和滑膜外肌腱片段反应的比较表明，滑膜内肌腱比 MCDB 105 中合成更多的蛋白聚糖、蛋白质和 DNA。滑膜外肌腱在两种介质中具有相似的基质成分和 DNA 合成率。滑膜内屈肌腱节段和滑膜外腓骨肌腱节段之间基质成分和 DNA 的合成存在显着差异的研究结果表明，滑膜内屈肌腱可能特别适应滑膜内环境提供的营养环境。这些数据得到了在有利于软骨组织培养的培养基中对滑膜内屈肌腱内纤维软骨节段的选择性成功刺激的支持。
Intrasynovial and extrasynovial flexor tendon grafts recently have been shown to have dissimilar patterns of cellular survival and host integration within the digital sheath. in an effort to determine if fundamental differences exist between these two types of tendons, we investigated the biochemical composition and cellular activity of intrasynovial and extrasynovial tendon segments of 12 adult mongrel dogs in short-term explant culture in MCDB 105 and in DMEM media. Proteoglycan, collagen and noncollagen protein synthesis and content and DNA synthesis were determined following culture in both media. Intrasynovial tendon segments cultured in MCDB 105 medium synthesized significantly less collagen, noncollagen protein, and DNA and had similar amounts of proteoglycans compared to extrasynovial tendons. Comparison of intrasynovial and extrasynovial tendon segment responses in DMEM medium showed that intrasynovial tendons synthesized more proteoglycan, protein, and DNA than they did in MCDB 105. Extrasynovial tendons had similar rates of matrix component and DNA synthesis in both media. Findings that the synthesis of matrix components and DNA between intrasynovial flexor and extrasynovial peroneal tendon segments differ significantly indicate that intrasynovial flexor tendons may be specially adapted to the nutritional milieu provided by an intrasynovial environment. These data are supported by the selective successful stimulation of fibrocartilaginous segments within intrasynovial Flexor tendons in media favored for the culture of cartilaginous tissue.