Microtubule sliding in reactivated flagella.
Microtubule sliding in reactivated flagella.
复制标题
重新激活的鞭毛中的微管滑动。
DOI：
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发表时间：
1982
期刊：
影响因子：
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通讯作者：
S. Kamimura
中科院分区：
文献类型：
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作者：
K. Takahashi;C. Shingyoji;S. Kamimura
本文介绍了近年来对海胆精子鞭毛中微管滑动的实验研究。一个本地的离子电渗应用ATP的Triton-extracted鞭毛elephant一个本地的弯曲反应，其形式是完全符合滑动微管模型的预测。在ATP存在下用胰蛋白酶处理脱膜鞭毛的片段所引发的微管滑动的电影分析揭示滑动的速度几乎是恒定的。这意味着速度不依赖于参与产生滑动力的动力蛋白臂的数量。表观滑动速度的分布表明，双联体之间的滑动速度没有差异。滑动速度取决于MgATP浓度的方式符合米氏动力学。胰蛋白酶处理的轴丝中的双联体的滑动速度接近于在相同MgATP浓度下重新激活的跳动鞭毛中相邻双联体之间发生相对滑动的最大速度。
Recent experimental studies of microtubule sliding in demembranated sea urchin sperm flagella are described. A local iontophoretic application of ATP to a Triton-extracted flagellum elicits a local bending response whose form is in exact conformity with the predictions of the sliding microtubule model. Cinematographic analysis of the microtubule sliding initiated by treating fragments of demembranated flagella with trypsin in the presence of ATP reveals that the speed of sliding is almost constant. This implies that the speed does not depend on the number of dynein arms participating in the generation of sliding force. The distribution of apparent sliding velocities indicates that there is no difference in sliding velocity among the doublets. The sliding velocity depends on MgATP concentration in a manner consistent with Michaelis-Menten kinetics. The sliding velocity of doublets in trypsin-treated axonemes is close to the maximum velocity of relative sliding taking place between adjacent doublets in beating flagella reactivated at the same MgATP concentration.