Dispersion of atmospheric fine particulate matters in simulated lung fluid and their effects on model cell membranes.
Dispersion of atmospheric fine particulate matters in simulated lung fluid and their effects on model cell membranes.
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DOI：
10.1016/j.scitotenv.2015.10.083
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发表时间：
2016-01
期刊：
影响因子：
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通讯作者：
Qiuhua Zhou;Lixin Wang;Zhaoyu Cao;Xuehua Zhou;Fan Yang;P. Fu;Zhenhua Wang;Jingtian Hu;Lei Ding
中科院分区：
文献类型：
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作者：
Qiuhua Zhou;Lixin Wang;Zhaoyu Cao;Xuehua Zhou;Fan Yang;P. Fu;Zhenhua Wang;Jingtian Hu;Lei Ding
采集大气细颗粒物(PM2.5)，研究其在模拟肺液(SLF)中的分散及其与模型细胞膜的相互作用。PM2.5可溶物中检出有机酸、NH_4~+、SO_4~(2+)、−和NO_3~(3-)−，从总质量中检出重金属。红外光谱表明，不溶组分中含有高岭石、碳酸钙、脂肪族碳、芳香环、羧基和羟基。蛋白质分散在SLF中的PM2.5中，导致较小的流体直径(Dh)和较慢的沉降速率。相反，磷脂提高了H值，加快了沉降速度。以巨大单层囊泡(GUV)和支撑类脂双层(SLB)为模型细胞膜。PM2.5附着并破坏了含有正电荷脂类的膜，而不是含有中性和负电荷脂类的膜，这是由显微镜和石英晶体微天平(QCM-D)监测的。膜上的阳离子位是PM2.5附着所必需的，但PM2.5含氧基团与磷脂基团之间的静电作用力和氢键的共同作用会破坏膜。我们的结果明确了蛋白质和磷脂在PM2.5扩散和转运中的作用，高度暗示PM2.5的健康危害与肺液中的生物分子和颗粒物表面群有关。
Atmospheric fine particulate matter (PM2.5) was collected to investigate its dispersion in simulated lung fluid (SLF) and its interaction with model cell membranes. Organic acids, NH4+, SO42 −and NO3−were detected in PM2.5soluble fraction, and heavy metals were detected from the total mass. The insoluble fraction contained kaolinite, CaCO3, aliphatic carbons, aromatic rings, carboxyl and hydroxyl groups reflected by the infrared spectra. Proteins dispersed PM2.5in SLF, resulted in smaller hydrodynamic diameter (dH) and slower sedimentation rate. Conversely, phospholipids increaseddHvalue and accelerated sedimentation rate. Giant unilamellar vesicles (GUVs) and supported lipid bilayers (SLBs) were used as model cell membranes. PM2.5adhered on and disrupted the membrane containing positively-charged lipids but not the membrane containing neutrally- and negatively-charged lipids, which was monitored by microscopy and a quartz crystal microbalance with dissipation (QCM-D). The cationic sites on membrane were necessary for PM2.5adhesion, but membrane should be disrupted by the combined action of electrostatic forces and hydrogen bonds between PM2.5oxygen containing groups and the lipid phosphate groups. Our results specified the roles of proteins and phospholipids in PM2.5dispersion and transport, highly suggested that the health hazard of PM2.5was related to the biomolecules in the lung fluid and the particle surface groups.