Placental development during early pregnancy in sheep: estrogen and progesterone receptor messenger RNA expression in pregnancies derived from in vivo-produced and in vitro-produced embryos
Placental development during early pregnancy in sheep: estrogen and progesterone receptor messenger RNA expression in pregnancies derived from in vivo-produced and in vitro-produced embryos
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DOI：
10.1016/j.domaniend.2015.05.003
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发表时间：
2015-10-01
影响因子：
2.1
通讯作者：
Grazul-Bilska, A. T.
中科院分区：
文献类型：
--
作者：
Reynolds, L. P.;Haring, J. S.;Grazul-Bilska, A. T.
性激素是包括胎盘在内的生殖组织中血管生成和生长的重要调节因子。在实验1中，为了研究性类固醇受体在妊娠早期的表达，在交配后14天至30天和发情后第10天(非妊娠对照组)收集了母体(角质细胞[CAR])和胎儿(胎膜[FM])胎盘组织。在EXP中2、为了验证辅助生殖技术会影响同一组性类固醇受体表达的假设，通过自然交配(NAT)或自然交配胚胎移植(NAT-ET)、体外受精(IVF)或体外激活(IVA)获得妊娠，并在第22天收集CAR和FM。在EXP中对于雌激素受体(ESR)1和2的CAR信使RNA(MRNA)的表达，核(N)孕激素受体(PGR)和膜(M)孕激素受体(PgR)α、β和伽马的表达受妊娠阶段的影响(P<；0.02)，Fm的ESR1、nPGR和mPGR的α、β和伽马(P<；0.03)也受到影响。在EXP中对于CAR，NAT-ET组、IVF组和IVA组ESRI和nPGRmRNA的表达均低于NAT组(P<0.001)。与NAT和NAT-ET相比，IVA组ESRI基因表达更强(P=0.10)，NAT-ET和IVF组GPER1表达更强(P<0.05)。这些结果建立了绵羊妊娠早期母体和胎儿胎盘性类固醇受体基因表达的正常模式，提示胎盘性类固醇受体的表达改变可能是辅助生殖技术后胎盘血管形成和生长不良的早期事件。(C)2015 Elsevier Inc.保留所有权利。
Sex steroids are important regulators of angiogenesis and growth in reproductive tissues, including the placenta. In experiment (exp.) 1, to examine the expression of a suite of sex steroid receptors throughout early pregnancy, maternal (caruncular [CAR]) and fetal (fetal membranes [FM]) placental tissues were collected on days 14 to 30 after mating and on day 10 after estrus (nonpregnant controls). In exp. 2, to examine the hypothesis that assisted reproductive technology would affect the expression of the same suite of sex steroid receptors, pregnancies were achieved through natural mating (NAT) or transfer of embryos from natural mating (NAT-ET), in vitro fertilization (IVF), or in vitro activation (IVA), and CAR and FM were collected on day 22. In exp. 1, for CAR messenger RNA (mRNA) expression of estrogen receptors (ESR) 1 and 2, nuclear (n) progesterone receptors (PGR) and membrane (m) PGR alpha, beta, and gamma were affected (P < 0.02) by pregnancy stage, as were ESR1, nPGR, and mPGR alpha, beta, and gamma for FM (P < 0.03). In exp. 2, for CAR, mRNA expression of ESRI and nPGR was decreased (P < 0.001) in NAT-ET, IVF, and IVA groups compared with NAT. For FM, mRNA expression of ESRI tended to be greater (P = 0.10) in the IVA group compared with NAT and NAT-ET, and GPER1 was greater (P < 0.05) in NAT-ET and IVF compared with NAT. These data establish the normal pattern of sex steroid receptor mRNA expression in maternal and fetal placenta during early pregnancy in sheep, and in addition, suggest that altered expression of placental sex steroid receptors may be an early event leading to poor placental vascularization and growth after assisted reproductive technology. (C) 2015 Elsevier Inc. All rights reserved.