Multimeric Near IR-MR Contrast Agent for Multimodal In Vivo Imaging.
Multimeric Near IR-MR Contrast Agent for Multimodal In Vivo Imaging.
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DOI：
10.1021/jacs.5b04509
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发表时间：
2015-07-22
影响因子：
15
通讯作者：
Meade TJ
中科院分区：
文献类型：
--
作者：
Harrison VS;Carney CE;MacRenaris KW;Waters EA;Meade TJ
体内成像应用通常需要多种成像模式，既需要高探头灵敏度，又需要出色的空间和时间分辨率。特别是，MR 和光学成像是一种有吸引力的组合，可用于确定分子和解剖信息。在此，我们描述了两种含有三种 Gd(III) 螯合物和 IR-783 染料部分的多聚体 NIR-MR 造影剂的合成和体内测试。一种试剂含有 PEG 连接体，另一种含有短烷基连接体。这些试剂以非凡的功效标记细胞，并且可以使用两种成像方式在体内进行检测。聚乙二醇化药物的生物分布在异种移植 MCF7 肿瘤中显示出可观察到的荧光，并且通过 MR 成像进行肾清除。
Multiple imaging modalities are often required for in vivo imaging applications that require both high probe sensitivity and excellent spatial and temporal resolution. In particular, MR and optical imaging are an attractive combination that can be used to determine both molecular and anatomical information. Herein, we describe the synthesis and in vivo testing of two multimeric NIR–MR contrast agents that contain three Gd(III) chelates and an IR-783 dye moiety. One agent contains a PEG linker and the other a short alkyl linker. These agents label cells with extraordinary efficacy and can be detected in vivo using both imaging modalities. Biodistribution of the PEGylated agent shows observable fluorescence in xenograft MCF7 tumors and renal clearance by MR imaging.