Recombinant human bone morphogenetic protein-2 promotes wound healing in rat periodontal fenestration defects
Recombinant human bone morphogenetic protein-2 promotes wound healing in rat periodontal fenestration defects
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DOI：
10.1177/00220345970760080801
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发表时间：
1997-08-01
影响因子：
7.6
通讯作者：
Hughes, FJ
中科院分区：
文献类型：
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作者：
King, GN;King, N;Hughes, FJ
尽管人们对使用骨形态发生蛋白（BMP）促进牙周再生很感兴趣，但对其对伤口愈合早期阶段的影响却知之甚少。本研究的目的是在牙周再生大鼠模型中研究重组人骨形态发生蛋白 2 (rhBMP-2) 对术后伤口愈合早期和完全愈合后（分别为 10 天和 38 天）的影响。在全身麻醉下，通过在 Wistar 大鼠下颌骨中形成的骨窗，小心地去除磨牙根颊侧的牙周韧带。根表面经过酸处理后，将 10μL 50μg/mL rhBMP-2 的胶原凝胶溶液放入试验动物手术造成的缺损处；在对照中，要么仅接受10μL量的胶原蛋白凝胶，要么不对缺陷进行治疗。手术后10天或38天处死动物并处理组织用于组织学检查。对横向 5 μm 切片进行染色，以鉴定新骨、牙骨质和胶原纤维的形成。在为期 10 天的研究组中，测试组第二磨牙和缺损区以外的新骨形成显着增加 (p < 0.02)，但没有证据表明强直增加。与对照相比，RhBMP-2 刺激的牙骨质生长面积是冠状面的两倍多（分别为 712 +/- 286 μ m(2) 和 258 +/- 57 μ m(2)）。结缔组织附着，包括胶原蛋白束的数量和宽度，在测试和对照中是相似的。术后 38 天，所有动物均完全愈合，没有任何强直迹象，并且测试组和对照组之间没有观察到显着差异。总之，单剂量的 rhBMP-2 增加了正常膜内骨形成的速度，并在早期伤口愈合过程中选择性地增强了冠状面的牙骨质形成。然而，rhBMP-2 在距缺损一定距离处诱导骨形成的发现表明，开发合适的递送系统以维持植入部位的 BMP-2 浓度以用于潜在的治疗用途非常重要。
Although there is considerable interest in the use of bone morphogenetic protein (BMP) to promote periodontal regeneration, little is known of its effects on the early stages of wound healing. The aim of this study was to investigate the effects of recombinant human bone morphogenetic protein 2 (rhBMP-2) on an early stage of post-operative wound healing and following complete healing (10 and 38 days, respectively) in a rat model of periodontal regeneration. The buccal aspects of molar roots were carefully denuded of their periodontal ligament through a bony window created in the mandibles of Wistar rats under general anesthesia. After the root surfaces were acid-conditioned, a 10-mu L quantity of 50 mu g/mL rhBMP-2 in a collagen gel solution was placed into the surgically created defect in test animals; in controls, either a 10-mu L quantity of only collagen gel was received, or the defect was untreated. Animals were killed 10 days or 38 days after surgery and the tissues processed for histological examination. Transverse 5-mu m sections were stained for the identification of new bone, cementum, and collagen fiber formation. In the 10-day study groups, new bone formation over the second molar and beyond the defect was significantly increased in the test group (p < 0.02), although there was no evidence of increased ankylosis. RhBMP-2 stimulated more than twice the area of cementum growth coronally compared with controls (712 +/- 286 mu m(2) and 258 +/- 57 mu m(2), respectively). Connective tissue attachment, including the number and width of collagen bundles, was similar in both test and controls. Complete healing without any evidence of ankylosis had occurred in all animals 38 days postoperatively, and no significant differences were observed between test and control groups. In conclusion, a single dose of rhBMP-2 increased the rate of normal intramembranous bone formation and selectively enhanced cementum formation coronally during early wound healing. However, the finding that rhBMP-2 induced bone formation at some distance from the defect suggests the importance of developing a suitable delivery system to maintain the concentration of BMP-2 at the site of implantation for potential therapeutic use.