Comprehensive analysis of cytokines in the follicular fluid and serum during in vitro fertilization and embryo transfer
Comprehensive analysis of cytokines in the follicular fluid and serum during in vitro fertilization and embryo transfer
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体外受精和胚胎移植过程中卵泡液和血清中细胞因子的综合分析
DOI：
10.11482/kmj-e202147167
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发表时间：
2021
期刊：
影响因子：
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通讯作者：
Koichiro SHIMOYA
中科院分区：
文献类型：
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作者：
Keiko MATSUMOTO;Takehiko MATSUYAMA;Shin OHIRA;Rumi NAKAZAWA;Yukiko HAZAMA;Takuya MORIYA;Ryo MATSUMOTO;Takafumi NAKAMURA;Koichiro SHIMOYA
体外受精（IVF）的妊娠率仍然很低，卵子的质量可能是一个促成因素。因此，对卵泡液中的细胞因子进行综合分析，以确定卵子质量的潜在标志物。选择因男性因素或未知病因接受IVF的女性的卵子（排除因女性因素接受治疗的卵子）进行细胞因子分析。对卵泡液进行全面的细胞因子/趋化因子分析。接下来，进行酶联免疫吸附试验（ELISA），用于定量142例随机选择的患者的血清和卵泡液中的白细胞介素（IL）-4，IL-17 A和IL-1 ra水平。综合分析显示，所有卵子等级的卵泡液中IL-4、IL-17 A和IL-1 ra水平存在显著差异。血清中的细胞因子水平揭示了卵子发育成胚泡的妇女和卵子没有发育成胚泡的妇女之间IL-17 A水平的显著差异。但卵泡液中细胞因子水平无显著差异。
Pregnancy rate for in vitro fertilization (IVF) remains low, for which the quality of ovum may be a contributing factor. Hence, comprehensive analysis of cytokines in follicular fluid was performed to identify potential markers of ovum quality. Ova from women undergoing IVF for male factors or unknown etiology, excluding those undergoing treatment for female factors, were selected for cytokine analysis. Comprehensive cytokine/chemokine analysis of follicular fluid was performed. Next, enzyme-linked immunosorbent assay (ELISA) for quantifying interleukin (IL)-4, IL-17A, and IL-1ra levels in the serum and follicular fluid of 142 randomly selected patients was conducted. The comprehensive analysis revealed significant differences in the levels of IL-4, IL-17A, and IL-1ra in the follicular fluid from all ovum grades. The cytokine levels in serum revealed a significant difference in IL-17A levels between women whose ova developed into blastocysts and women whose ova did not. However, no significant differences were found in the cytokine levels of follicular fluid.