Antibodies against human Toll-like receptors (TLRs): TLR distribution and localization in human dendritic cells
Antibodies against human Toll-like receptors (TLRs): TLR distribution and localization in human dendritic cells
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DOI：
10.1179/096805105x67292
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发表时间：
2005-12-01
期刊：
影响因子：
--
通讯作者：
Matsumoto, M
中科院分区：
文献类型：
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作者：
Seya, T;Funami, K;Matsumoto, M
我们已经产生了针对人Toll样受体（TLR）的单克隆抗体（mAb）。这些单克隆抗体识别细胞外结构域富含亮氨酸的重复序列的天然构象，因此，适用于免疫沉淀，流式细胞术分析和免疫染色。使用这些单克隆抗体，我们确定了TLR在各种人类细胞群体中的分布。识别细菌组分的人TLR，特别是TLR-1、TLR-2、TLR-4和TLR-6，位于细胞表面上，并在骨髓和单核细胞衍生的树突状细胞（DC）中表达，但不在浆细胞样DC（pDC）中表达。人TLR-3存在于髓样DC中推定的内体中。因此，人髓样DC亚群具有独特且独特的TLR库。这些单克隆抗体将用于检测人TLR在各种细胞和器官中的定位和分布。人类TLR的功能研究也是可行的，因为它们中的某些是功能阻断mAb。
We have produced monoclonal antibodies (mAbs) against human Toll-like receptors (TLRs). These mAbs recognize the natural conformation of the extracellular domain leucine-rich repeats and, thereby, are suitable for immunoprecipitation, flow cytometric analysis and immunostaining. Using these mAbs, we determined the distribution of TLRs in a variety of human cell populations. Human TLRs that recognize bacterial components, particularly TLR-1, TLR-2, TLR-4 and TLR-6, reside on the cell surface and are expressed in myeloid and monocyte-derived dendritic cells (DCs) but not in plasmacytoid DCs (pDCs). Human TLR-3 resides in putative endosomes in myeloid DCs. Thus, the human myeloid DC subset harbors a unique and distinctive TLR repertoire. These mAbs will be useful to test the localization and distribution of human TLRs in a variety of cells and organs. Functional studies of human TLRs will also be feasible since certain of them are function-blocking mAbs.