The effect of dietary restriction on mitochondrial protein density and flight muscle mitochondrial morphology in Drosophila
The effect of dietary restriction on mitochondrial protein density and flight muscle mitochondrial morphology in Drosophila
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DOI：
10.1093/gerona/61.1.36
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发表时间：
2006-01-01
影响因子：
5.1
通讯作者：
Partridge, L
中科院分区：
文献类型：
--
作者：
Magwere, T;Goodall, S;Partridge, L
饮食限制（DR）延长了不同生物体的寿命，并可能通过减少线粒体活性氧（ROS）的产生来实现。然而，测量ROS生产从分离的线粒体的生物体进行DR产生不一致的结果。在果蝇Drosophila中，DR不减少来自分离的线粒体的ROS的产生。在这项研究中，我们使用果蝇来测试DR是否会降低线粒体密度。我们使用（a）线粒体酶的活性和（B）电子显微镜评估了DR和对照饮食的果蝇的线粒体密度。这两种方法都没有显示DR对线粒体密度的整体影响，但是，DR和对照果蝇之间的线粒体酶活性和形态学差异显着。我们的结论是，寿命延长的DR在果蝇是不介导的线粒体密度的减少。如果果蝇中的DR通过减少ROS的产生来延长寿命，那么它是通过仅在体内起作用的机制来实现的。
Dietary restriction (DR) extends life span in diverse organisms and may do so by attenuating production of mitochondrial reactive oxygen species (ROS). However, measurements of ROS production from isolated mitochondria of organisms subjected to DR have produced inconsistent results. In the fruit fly Drosophila, DR does not reduce production of ROS from isolated mitochondria. In this study, we used Drosophila to test whether DR lowered mitochondrial density. We assessed mitochondrial densities of flies on DR and Control diets using (a) the activities of mitochondrial enzymes and (b) electron microscopy. Both methods showed no overall effect of DR on mitochondrial density; however, mitochondrial enzyme activities and morphology differed significantly between DR and Control flies. We concluded that life-span extension by DR in Drosophila is not mediated through a reduction in mitochondrial density. If DR in Drosophila extends life span by reducing ROS production, then it does so through mechanisms that operate only in vivo.