Mago Nashi and Tsunagi/Y14, respectively, regulate Drosophila germline stem cell differentiation and oocyte specification.
Mago Nashi and Tsunagi/Y14, respectively, regulate Drosophila germline stem cell differentiation and oocyte specification.
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DOI：
10.1016/j.ydbio.2007.06.007
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发表时间：
2007-08
影响因子：
2.7
通讯作者：
D. Parma;P. Bennett;R. Boswell
中科院分区：
文献类型：
--
作者：
D. Parma;P. Bennett;R. Boswell
一个蛋白质复合物组成的MagoNashi和Tsunagi/Y14是必需的，以建立主要的身体轴和本地化的原始生殖细胞决定因素在果蝇卵子发生。Mago Nashi：Tsunagi/Y14异二聚体也是外显子连接复合物（EJC）的核心，EJC是一种组装在剪接mRNA上的多蛋白复合物。在以前的研究中，功能降低的等位基因magonashi和tsunagi/Y14被用来描述基因在卵子发生中的作用。在这里，我们调查mago nashi和tsunagi/Y14使用无效等位基因和克隆分析。缺乏mago nashi功能的生殖系克隆分裂但不能分化。空的生殖系干细胞在至少11天的时间内产生克隆，这表明空的生殖系干细胞不是干细胞自我更新所必需的。然而，缺乏tsunagi/Y14功能的生殖系干细胞与野生型无法区分。此外，在tsunagi/Y14无效生殖系包囊中，中心体和卵母细胞特异性组分未能集中在单个细胞内，卵母细胞的命运不限于单个细胞。总之，我们的研究结果表明，不仅是需要的生殖干细胞分化，但令人惊讶的是，在这个过程中，mago nashi功能独立的tsunagi/Y14。另一方面，Tsunagi/Y14对于将卵母细胞命运限制在单个细胞中是必不可少的，并且可能在此过程中与mago nashi一起起作用。
A protein complex consisting of Mago Nashi and Tsunagi/Y14 is required to establish the major body axes and for the localization of primordial germ cell determinants during Drosophila melanogaster oogenesis. The Mago Nashi:Tsunagi/Y14 heterodimer also serves as the core of the exon junction complex (EJC), a multiprotein complex assembled on spliced mRNAs. In previous studies, reduced function alleles of mago nashi and tsunagi/Y14 were used to characterize the roles of the genes in oogenesis. Here, we investigated mago nashi and tsunagi/Y14 using null alleles and clonal analysis. Germline clones lacking mago nashi function divide but fail to differentiate. The mago nashi null germline stem cells produce clones over a period of at least 11 days, suggesting that mago nashi is not necessary for stem cell self-renewal. However, germline stem cells lacking tsunagi/Y14 function are indistinguishable from wild type. Additionally, in tsunagi/Y14 null germline cysts, centrosomes and oocyte-specific components fail to concentrate within a single cell and oocyte fate is not restricted to a single cell. Together, our results suggest not only that mago nashi is required for germline stem cell differentiation but that surprisingly mago nashi functions independently of tsunagi/Y14 in this process. On the other hand, Tsunagi/Y14 is essential for restricting oocyte fate to a single cell and may function with mago nashi in this process.