Cloning and expression analysis of a new chalcone isomerase gene during flowering in safflower
Cloning and expression analysis of a new chalcone isomerase gene during flowering in safflower
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红花花期查耳酮异构酶新基因的克隆及表达分析
DOI：
10.3906/bot-1809-25
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发表时间：
2019-01-01
影响因子：
1.8
通讯作者：
Wu, Qinghua
中科院分区：
文献类型：
--
作者：
Ren, Chaoxiang;Tang, Xiaohui;Wu, Qinghua
红花（Carthamus tinctorius L.）由于其改善脑血流的能力而被广泛用于传统中医。红花的主要活性成分是黄酮类化合物。迄今为止，很少有黄酮类化合物生物合成基因已被克隆从该物种。在我们前期的研究中，我们筛选了四个查尔酮异构酶基因。本研究从红花cDNA文库中克隆了一个新的查尔酮异构酶基因（CtCHI-N）。克隆了CtCHI-N的696-bp cDNA，并随后分析了其序列。重组蛋白在原核表达系统中成功表达，并研究了CtCHI-N在不同组织和开花不同时期的表达。结果表明，CtCHI-N基因具有组织特异性表达，在开花初期表达量最高。此外，还测定了不同开花阶段的类黄酮，发现CtCHI-N的表达与开花期间类黄酮的生物合成相关。本实验成功克隆了CtCHI-N基因，表达分析表明CtCHI-N基因可能影响红花开花过程中类黄酮的合成。
Flowers of safflower (Carthamus tinctorius L.) have been widely used in traditional Chinese medicine because of their ability to improve cerebral blood flow. The major active ingredients in safflower are flavonoids. To date, few flavonoid biosynthesis genes have been cloned from this species. In our previous research, four isoform chalcone isomerase genes were screened. In this study, a new chalcone isomerase gene (designated as CtCHI-N) was cloned from a safflower cDNA library. A 696-bp cDNA for CtCHI-N was cloned, and its sequence was subsequently analyzed. The recombinant protein was successfully expressed in a prokaryotic expression system, and the expression of CtCHI-N in different tissues and at different stages of flowering was investigated. The results showed that CtCHI-N was expressed in a tissue-specific manner, and the greatest expression level occurred during early flowering. In addition, flavonoids at different stages of flowering were also measured, and the expression of CtCHI-N was found to correlate with flavonoid biosynthesis during flowering. CtCHI-N was successfully cloned in the experiment, and expression analysis revealed that CtCHI-N might influence flavonoid biosynthesis during flowering in safflower.