Nisin-selectable food-grade secretion vector for Lactococcus lactis
Nisin-selectable food-grade secretion vector for Lactococcus lactis
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用于乳酸乳球菌的乳链菌肽选择性食品级分泌载体
DOI：
10.1007/s10529-010-0503-6
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发表时间：
2011-04-01
影响因子：
2.7
通讯作者：
Saris, Per E. J.
中科院分区：
文献类型：
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作者：
Li, Ruiqing;Takala, Timo M.;Saris, Per E. J.
从原料奶中分离到一株乳链菌素抗性乳酸菌TML01。鉴定出一个与乳链菌素抗性基因NSR有99%同源性的基因。构建了基于nsr基因的食品级分泌表达载体pLEB690(3746bp)，并在乳酸乳杆菌中表达和分泌了儿茶素ACh基因PapA，初步筛选出了高达5μg Nisin/ml的nsr基因。因此，pLEB690是一种功能强大的食品级分泌载体，具有潜在的食品工业应用价值。
A nisin-resistant Lactococcus lactis strain TML01 was isolated from crude milk. A gene with 99% homology to the nisin-resistance gene, nsr, was identified. The food-grade secretion plasmid, pLEB690 (3746 bp), was constructed based on this novel nsr gene enabling primary selection with up to 5 μg nisin/ml. The functionality of pLEB690 as a secretion vector was shown by expressing and secreting the pediocin AcH gene papA in L. lactis. pLEB690 is therefore, a functional food-grade secretion vector potentially useful for the food industry.