A large family of genes that share homology with CLE domain in Arabidopsis and rice
A large family of genes that share homology with CLE domain in Arabidopsis and rice
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DOI：
10.4161/psb.3.5.5344
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发表时间：
2008-05
影响因子：
2.9
通讯作者：
S. Sawa;Atsuko Kinoshita;S. Betsuyaku;H. Fukuda
中科院分区：
文献类型：
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作者：
S. Sawa;Atsuko Kinoshita;S. Betsuyaku;H. Fukuda
31个CLAVATA3/胚乳周围区(ESR)相关(CLE)蛋白在拟南芥基因组中编码，它们被认为是含有两个羟基脯氨酸残基的十二肽。26个合成的CLE多肽，对应于31个CLE基因的预测产物，在拟南芥和水稻中进行了检测。19个CLE多肽诱导根分生组织的消耗，导致拟南芥和水稻的短根表型，而不影响水稻的顶端分生组织。数据库搜索在水稻基因组中发现了47个可能的CLE基因。其中3个水稻CLE基因OsCLE502、OsCLE504和OsCLE506编码具有多个CLE结构域的CLE蛋白，这些结构域在拟南芥基因组中是不存在的，并且在这些CLE结构域之间发现了多个多脯氨酸区域。这些结果表明，在每种植物中，CLE功能是保守的和/或不同的。
Thirty-one CLAVATA3/ENDOSPERM SURROUNDING REGION (ESR)-related (CLE) proteins are encoded in the Arabidopsis genome, and they are supposed to function as dodecapeptides with two hydroxyproline residues. Twenty-six synthetic CLE peptides, corresponding to the predicted products of the 31 CLE genes, were examined in Arabidopsis and rice. Nineteen CLE peptides induced root meristem consumption, resulting in the short root phenotype in Arabidopsis and rice, whereas no CLE peptides affected the shoot apical meristem in rice. Database searches revealed 47 putative CLE genes in the rice genome. Three of the rice CLE genes, OsCLE502, OsCLE504 and OsCLE506, encode CLE proteins with multiple CLE domains, which are not found in the Arabidopsis genome, and polyproline region was found between these CLE domains. These results indicate conserved and/or diverse CLE functions in each plant species.