A mitogen-activated protein kinase gene (MGV1) in Fusarium graminearum is required for female fertility, heterokaryon formation, and plant infection
A mitogen-activated protein kinase gene (MGV1) in Fusarium graminearum is required for female fertility, heterokaryon formation, and plant infection
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DOI：
10.1094/mpmi.2002.15.11.1119
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发表时间：
2002-11-01
影响因子：
3.5
通讯作者：
Xu, JR
中科院分区：
文献类型：
--
作者：
Hou, ZM;Xue, CY;Xu, JR
禾谷镰刀菌（Fusarium graminearum）是世界上许多地区小杂粮和玉米的重要病原菌。受感染的谷物通常被对人类和动物有害的真菌毒素污染。在过去的十年中，禾谷镰刀菌在小麦和大麦中引起了几次严重的头黑星病流行。为了了解调控真菌发育和致病性的分子机制，我们分离并鉴定了一个MAP激酶基因MGV1，该基因与稻瘟病菌MPS1基因高度同源。MGV1基因在禾谷麦胚的产孢过程中起重要作用，但在有性生殖过程中对雌性育性起重要作用。mgv1缺失突变体在液体培养基中生长正常，但在固体培养基中生长减少。mgv1突变体的菌丝细胞壁较弱，对细胞壁降解酶高度敏感。有趣的是，当测试异核体形成时，mgv 1突变体是自交不亲和的，并且它们的毒力大大降低。突变体在接种的小麦上积累多孢霉素真菌毒素的能力也大大降低。我们的数据表明，MGV1在E graminearum是参与多个发展过程有关的有性生殖，植物感染，细胞壁完整性。
Fusarium graminearum is an important pathogen of small grains and maize in many areas of the world. Infected grains are often contaminated with mycotoxins harmful to humans and animals. During the past decade, E graminearum has caused several severe epidemics of head scab in wheat and barley. In order to understand molecular mechanisms regulating fungal development and pathogenicity in this pathogen, we isolated and characterized a MAP kinase gene, MGV1, which is highly homologous to the MPS1 gene in Magnaporthe grisea. The MGV1 gene was dispensable for conidiation in E graminearum but essential for female fertility during sexual reproduction. Vegetative growth of mgv1 deletion mutants was normal in liquid media but reduced on solid media. Mycelia of the mgv1 mutants had weak cell walls and were hypersensitive to cell wall degrading enzymes. Interestingly, the mgv1 mutants were self-incompatible when tested for heterokaryon formation, and their virulence was substantially reduced. The ability of the mutants to accumulate trichothecene mycotoxins on inoculated wheat was also greatly reduced. Our data suggest that MGV1 in E graminearum is involved in multiple developmental processes related to sexual reproduction, plant infection, and cell wall integrity.