Multicolor stimulated Raman scattering microscopy with a rapidly tunable optical parametric oscillator.
Multicolor stimulated Raman scattering microscopy with a rapidly tunable optical parametric oscillator.
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带有快速可调光学参量振荡器的多色受激拉曼散射显微镜。
DOI：
10.1364/ol.38.000145
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发表时间：
2013-01-15
期刊：
影响因子：
3.6
通讯作者：
Xie XS
中科院分区：
文献类型：
--
作者：
Kong L;Ji M;Holtom GR;Fu D;Freudiger CW;Xie XS
受激拉曼散射（SRS）显微镜允许基于振动光谱的无标记化学成像。皮秒激光器的窄带激发产生最高的信号水平，并使成像速度达到视频速率，但与宽带（多路复用）激发相比，牺牲了样品中具有重叠频带的化学物质。我们开发了一种快速可调皮秒光参量振荡器与电光可调Lyot滤波器，并展示了多色SRS显微镜同步线的波长调谐，以避免由于样品移动的光谱伪影。我们显示了三种不同类型的聚合物珠和活的HeLa细胞与移动的细胞内脂滴的灵敏成像。
Stimulated Raman scattering (SRS) microscopy allows label-free chemical imaging based on vibrational spectroscopy. Narrowband excitation with picosecond lasers creates the highest signal levels and enables imaging speeds up to video-rate, but sacrifices chemical specify in samples with overlapping bands compared to broadband (multiplex) excitation. We develope a rapidly tunable picosecond optical parametric oscillator with an electro-optical tunable Lyot filter, and demonstrate multi-color SRS microscopy with synchronized line-by-line wavelength tuning to avoid spectral artifacts due to sample movement. We show sensitive imaging of three different kinds of polymer beads and live HeLa cells with moving intracellular lipid droplets.