On the mechanism of erythropoietin-induced differentiation. 13. The role of sialic acid in erythropoietin action.
On the mechanism of erythropoietin-induced differentiation. 13. The role of sialic acid in erythropoietin action.
复制标题
促红细胞生成素诱导分化的机制研究。
DOI：
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发表时间：
1974
影响因子：
4.8
通讯作者：
J. Eliason
中科院分区：
文献类型：
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作者：
E. Goldwasser;C. Kung;J. Eliason
促红细胞生成素是一种诱导正常红细胞发育的糖蛋白，每摩尔含有16 ~ 18个唾液酸残基。在体内试验时，脱水会导致生物活性完全丧失。当测定在体外进行时，asialoerythropo生成素具有充分的活性，或者当测定在低水平的激素时，其活性约为天然激素的三倍。活性丧失可以通过肝脏从循环中去除亚洲糖蛋白来解释(Morell, a.g., Irvine, r.a., Sternlieb， I., Scheinberg, i.h., and Ashwell， G.（1968）。化学。243,155)。将Asialoorosomucoid或水苏糖注射到实验动物体内，作为竞争对手，在脱水后，体内红细胞生成素的原始活性可达25%至35%。用半乳糖氧化酶治疗asialo红细胞生成素可使原活性恢复45%，证实了早期的发现（Lukowsky， W. A.，和Painter， R. H.(1972)）。生物化学学报，2009,5)。亚洲促红细胞生成素对热变性和胰蛋白酶的作用比天然激素更敏感。我们的数据表明，末端唾液酸是化学和生物稳定性所必需的，但不是对骨髓靶细胞的作用。asialalohormone增强了对靶细胞的活性。
Abstract Erythropoietin, a glycoprotein that induces normal erythrocyte development, has 16 to 18 sialic acid residues per mole. Desialation results in complete loss of biological activity when it is assayed in vivo. When the assay is done in vitro asialoerythropoietin has full activity, or when assayed at low levels of hormones is about three times more active than the native hormone. The loss of activity can be explained by the hepatic removal of asialoglycoproteins from the circulation (Morell, A. G., Irvine, R. A., Sternlieb, I., Scheinberg, I. H., and Ashwell, G. (1968) J. Biol. Chem. 243, 155). Asialoorosomucoid or stachyose injected into assay animals act as competitors and permit 25 to 35% of the original activity of erythropoietin to be seen in vivo after desialation. Treatment of asialoerythropoietin with galactose oxidase results in recovery of 45% of the original activity, confirming an earlier finding (Lukowsky, W. A., and Painter, R. H. (1972) Can. J. Biochem. 50, 909). Asialoerythropoietin is more sensitive to heat denaturation and trypsin action than the native hormone. Our data indicate that terminal sialic acid is required for chemical and biological stability but not for action on target cells of the bone marrow. The asialohormone has enhanced activity towards the target cells.