AtPDR12 contributes to lead resistance in arabidopsis
AtPDR12 contributes to lead resistance in arabidopsis
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DOI：
10.1104/pp.104.058107
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发表时间：
2005-06-01
期刊：
影响因子：
7.4
通讯作者：
Lee, Y
中科院分区：
文献类型：
--
作者：
Lee, M;Lee, K;Lee, Y
拟南芥 (Arabidopsis thaliana) 含有约 130 个 ATP 结合盒 (ABC) 蛋白，这些蛋白可能有助于多种物质的运输，包括有毒物质。然而，在大多数情况下，ABC 转运蛋白的底物仍然未知。我们测试了哪种 ABC 转运蛋白参与铅 [Pb(II)] 的解毒。在众多测试中，我们发现只有AtPDR12在Pb(II)处理的拟南芥的芽和根中的信息水平增加，表明它可能参与Pb(II)的解毒。 AtPDR12-敲除植物（atpdr12）被用来进一步测试这种可能性。在含 Pb(II) 的培养基中，atpdr12 植物比野生型植物生长较差，且 Pb 含量较高。相比之下，AtPDR12过表达的拟南芥植物比野生型植物对Pb(II)具有更强的抗性并且具有更低的Pb含量。突变表型及其 Pb 含量，以及 GFP：AtPDR12 融合蛋白在质膜上的定位表明，AtPDR12 起到泵的作用，从细胞质中排除 Pb(II) 和/或含有 Pb(II) 的有毒化合物。通过向生长培养基中添加丁硫氨酸亚砜亚胺来抑制谷胱甘肽合成加剧了 atpdr12 植物的 Pb(II) 敏感表型，这与与 AtPDR12 依赖性机制并行运行的谷胱甘肽依赖性解毒机制一致。因此，我们推测 AtPDR12 是一种 ABC 转运蛋白，有助于拟南芥对 Pb(II) 的抗性。
Arabidopsis ( Arabidopsis thaliana) contains about 130 ATP- binding cassette ( ABC) proteins, which are likely to contribute to the transport of diverse materials, including toxic substances. However, the substrates of ABC transporters remain unknown in most cases. We tested which ABC transporter is involved in detoxification of lead [ Pb( II)]. Among the many tested, we found that the message level of only AtPDR12 increased in both shoots and roots of Pb( II)- treated Arabidopsis, suggesting that it may be involved in the detoxification of Pb( II). AtPDR12- knockout plants ( atpdr12) were used to further test this possibility. In Pb( II)- containing medium, atpdr12 plants grew less well and had higher Pb contents than those of wild- type plants. In contrast, AtPDR12- overexpressing Arabidopsis plants were more resistant to Pb( II) and had lower Pb contents than wild- type plants. The mutant phenotypes and their Pb contents, as well as the localization of the GFP: AtPDR12 fusion protein at the plasma membrane, suggest that AtPDR12 functions as a pump to exclude Pb( II) and/ or Pb( II)- containing toxic compounds from the cytoplasm. Inhibition of glutathione synthesis by addition of buthionine sulfoximine to the growth medium exacerbated the Pb( II)- sensitive phenotype of atpdr12 plants, consistent with a glutathione- dependent detoxification mechanism operating in parallel with an AtPDR12- dependent mechanism. Thus, we propose that AtPDR12 is an ABC transporter that contributes to Pb( II) resistance in Arabidopsis.